摘要
目的研究姜黄素水解衍生物对氧化型低密度脂蛋白促牛主动脉平滑肌细胞增殖和对牛血管平滑肌细胞膜低密度脂蛋白受体表达的影响。方法用盐酸加三氯化铝水解姜黄素,获得姜黄素水解衍生物;用氧化型低密度脂蛋白培养牛血管平滑肌细胞建立促增殖模型,MTT比色法观察姜黄素水解衍生物对牛血管平滑肌细胞增的抑制作用;流式细胞仪检测血管平滑肌细胞膜上低密度脂蛋白受体表达的影响;采用逆转录聚合酶链反应检测c-myc的表达。结果姜黄素水解衍生物对10mg/L氧化型低密度脂蛋白培养的血管平滑肌细胞增殖有明显的抑制作用,高中低剂量组的抑制率分别为46%、37%和22%(P<0.01或0.05);对血管平滑肌细胞膜上的低密度脂蛋白受体的表达均有明显的上调作用,高中低剂量组低密度脂蛋白受体表达率分别比对照组高33.85%、17.95%和5.75%(P<0.01或0.05);能够下调c-myc的表达。结论姜黄素水解衍生物能明显抑制牛主动脉平滑肌细胞增殖,上调低密度脂蛋白受体的表达,从而延缓动脉粥样硬化的发生发展。
Aim To study the effects on the proliferafion of ramification of Curcumin hydrolyzed by HC1 （RCH） onthe bovine vascular smooth muscle cell （VSMC） and the expression of low density lipoprotein receptor （LDLR）. Method Curcumin was hydrolyzed by HC1, the proliferation models of the bovine VSMC were stimulated by ox-LDL, MTT was used to observe its effects on proliferation of the bovine VSMC. FCM was used to Observe its effects of expression of LDLR. The c-myc mRNA was measured by RT-PCR. Results Ramification of Curcumin Hydrolyzed have obvious effect on anti-proliferation of VSMC stimulated by 10 mg/L ox-LDL （ P 〈 0.01 ）. And it can obviously increase the expression of LDLR of VSMC and down-regulate the expression of c-myc. Conclusion Ramification of Curcumin hydrolyzed have obvious effect on anti-proliferation of VSMC, and it can increase the expression of LDLR. That may be the mechanism of delaying the development of arteriosclerosis.
出处
《中国动脉硬化杂志》
CAS
CSCD
2007年第4期261-264,共4页
Chinese Journal of Arteriosclerosis
基金
浙江省自然科学基金(202015)
