摘要
利用26SrDNAD1/D2区序列分析法对中国工业微生物菌种保藏管理中心(CICC)保藏的22株酿酒酵母(Saccharomycescerevisiae)和1株威尔酵母(S.willianus)进行了复核鉴定,通过序列比对及构建系统发育树分析后,结果显示:21株菌株与原名称一致,与酿酒酵母CBS1171T序列相似性在99.0%以上;CICC1313原定名为威尔酵母,此次鉴定结果为酿酒酵母,与酿酒酵母CBS1171T序列相似性为99.8%;CICC1859与异常毕赤酵母(Pichiaanomala)CBS5759T相似性为100%,鉴定为异常毕赤酵母。进一步对酿酒酵母与酵母属内其他种之间的发育关系进行了分析,通过构建酵母属内各菌种模式株的26SrDNAD1/D2区系统发育树,发现酿酒酵母与属内其他菌种间差异均大于1%,表明26SrDNAD1/D2区域序列分析方法能够应用于酿酒酵母的分子生物学鉴定。
22 Saccharomyces cerevisiae strains and 1 S. willianus were identified by 26S rDNA D 1/D2 domain sequences analysis, which were deposited in China Center oflndustrial Culture Collection (CICC). The similarities of 21 strains with the type strain ofS. cerevisiae C BS 1171T are from 99.0% to 100%. The result indicated that the 21 strains support the original identification resuhs;CICC 1313, which was named as S. willianus originally, was clustered with S. cerevisiae closely in the phlogcnetic tree and exibited 99.8% similarity with type strain CBS 117 IT. The similarity of CICC 1859 and Pichia anomala CBS 5759T was 100%. The relationship of 26S rDN A gene D1/D2 domain sequences among Saccharomyces' strains was analyzed furthermore and the divergence of S. cerevisiae with the other Saecharomyces' strains were greater than 1%, which indicated 26S rD N A D 1/D2 domain sequences analysis is fit for S. cerevisiae identification.
出处
《酿酒》
CAS
2007年第1期37-39,共3页
Liquor Making
基金
国家科技基础平台建设项目(2005DKA21204)