摘要
以结缕草叶片为外植体进行离体再生研究.结果表明:叶片愈伤组织诱导的最佳培养基是MS+1.5mg·L-12,4-二氯苯氧乙酸(2,4-D)+0.5mg·L-16-苄基腺嘌呤(6-BA)+26g·L-1蔗糖+5.0g·L-1琼脂,诱导率为65.36%,无性世代增殖倍数高达9.56;正交试验和方差分析筛选出不定芽分化最佳培养基为MS+3.0mg·L-16-BA+0.5mg·L-1萘乙酸(NAA)+1.5mg·L-1AgNO3+35g·L-1蔗糖+6.5g·L-1琼脂,分化率为67.30%,增殖系数为8.67;生根培养基选用1/4MS+0.2mg·L-1吲哚丁酸(IBA)+0.3mg·L-1NAA+28g·L-1蔗糖+4.5g·L-1琼脂,生根率为90.00%.
Regeneration system of Zoysia japonica Steud.in vitro was studied with leaves as explants.The results showed that optimal medium for callus induction was MS＋1.5 mg·L^-1 2,4-dichlorophenoxyacetic acid（2,4-D）＋0.5 mg·L^-1 6-benzyladenine（6-BA）＋26 g·L^-1 sucrose＋5.0 g·L^-1 agar,with the induction frequency of 65.36%,multiplied times of clone generations reached 9.56.Best bud differentiation and bud multiplying medium was MS＋3.0 mg·L^-1 6-BA ＋0.5 mg·L^-1 α-naphthaleneacetic acid（NAA）＋1.5 mg·L^-1 AgNO3＋35 g·L^-1 sucrose＋6.5 g·L^-1 agar which was sifted through orthogonal experiment and variance analysis,with bud differentiation rate of 67.30% and multiplying coefficient of 8.67.The best medium for rooting induction was 1/4 MS＋0.2 mg·L^-1 indole-3-butyric acid（IBA）＋0.3 mg·L^-1 NAA＋28 g·L^-1 sucrose＋4.5 g·L^-1 agar,the rooting rate was 90.00%.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2007年第5期507-513,共7页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
河南省重大科技攻关资助项目(0524050006).
关键词
结缕草
叶片
离体再生
正交设计
方差分析
Zoysia japonica Steud.
leaves
in vitro regeneration
orthogonal design
variance analysis
