摘要
为了研究过敏性紫癜（ＡＰ）的发病机理中是否有免疫遗传因素参与，采用国际通用的ＮＩＨ标准微量淋巴细胞毒试验方法检测４０例ＡＰ患者的ＨＬＡ－Ⅰ类抗原，并与１００例北方汉族正常人ＨＬＡ－Ⅰ类抗原频率进行比较。利用聚合酶链反应－序列特异性引物技术（ＰＣＲ－ＳＳＰ）对其中３０例ＡＰ患者进行ＨＬＡ－Ⅱ类基因分型，并与１０４例北方汉族正常人的ＨＬＡ－Ⅱ类基因频率进行了比较。发现ＡＰ患者ＨＬＡ－Ａ３０＋３１、Ｂ１３、Ｂ３５、Ｂ４０抗原频率较对照组明显增高（Ａ３０＋３１：Ｐｃ＜０．０１，ＲＲ＝７．９７；Ｂ１３∶ＰＣ＜０．０１，ＲＲ＝６．００，Ｂ３５∶Ｐｃ＜１０－５，ＲＲ＝１０．４０；Ｂ４０∶Ｐｃ＜０．０５，ＲＲ＝３．８５）。ＨＬＡ－ＤＲ１０基因频率在ＡＰ患者较正常对照组明显增高（ＤＲ１０∶Ｐｃ＜１０－５，ＲＲ＝２１．８８），而ＨＬＡ－ＤＱ３、ＤＱ６基因频率较正常对照组明显降低（ＤＱ３∶Ｐｃ＜１０－５，ＲＲ＝０．１３；ＤＱ６∶Ｐｃ＜０．０５，ＲＲ＝０．２３）。提示ＡＰ与ＨＬＡ－Ａ３０＋３１、Ｂ１３、Ｂ３５、Ｂ４０、ＤＲ１０正相关，与ＨＬＡ－ＤＱ３ＤＱ６负相关。
The objective of this study was to investigate the possible involvement of hereditary factors in the pathogenesis of anaphylactoid purpura. Forty patients with anaphylactoid purpura and 100 normal controls in the same gengraphic region were tested for HLA class I antigen frequencies by using the NIH standard microlymphocytotoxicity assay. In addition, 30 of these patients were genotyped, and their HLA class Ⅱ antigen gene frequencies were compared with those of 104 normal controls by using polymerase chain reaction sequence specific primer (PCR SSP). The results showed that HLA A 30+31 ,B 13 ,B 35 ,B 40 antigen frequencies were significantly higher in patients with anaphylactoid purpura than in the controls(A 30+31 ∶ P c<0.01, RR=7.97; B 13 ∶ P c<0.01, RR=6.00; B 35 ∶ P c<10 -5 ,RR=10.40; B 40 ∶ P c<0.05,RR=3.85). While HLA DR 10 gene frequency was markedly higher in patients than in controls(DR 10 ∶ P c<10 -5 , RR=21.88),HLA DQ 3,DQ 6 gene frequencies were significantly lower in the patients (DQ 3∶ P c<10 -5 ,RR=0.13; DQ 6∶ P c<0.05,RR=0.23). These suggest that anaphylactoid purpura be positively related to HLA A 30+31 ,B 13 ,B 35 ,B 40 and DR 10 , and be negatively related to HLA DQ 3,DQ 6.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
1997年第2期79-81,共3页
Chinese Journal of Medical Genetics
关键词
过敏性紫癜
聚合酶链反应
HLA
汉族
Anaphylactoid purpura Human leucocyte antigen Polymerase chain reaction
