摘要
目的:对甲苯胺红不加热血清试验(TRUST)、酶联免疫吸附试验(TP-ELISA)检测方法的有效性进行综合分析,选择适合采供血机构筛查梅毒的方法。方法:用TRUST、TP-ELISA方法对献血者进行梅毒筛查,阳性标本用梅毒螺旋体明胶凝集试验(TPPA)方法确证。结果:TRUST方法灵敏度为39.02%,假阳性率为3.03%,漏检率为60.98%;ELISA方法灵敏度为98.73%,假阳性率为20.59%,漏检率为1.22%。结论:TRUST、ELISA两种试剂作为献血员筛查梅毒的首选组合方案,更有利于血液质量安全。
Objective To select the best screening method suiting for blood donors by assessing the validity of TRUST, ELISA and TPPA, Methods TRUST and ELISA were used to screen patients with syphilis from blood donors, and TPPA was used to confirmed positive results detected by ELISA or TRUST. Results The sensitivity,false positive rate and false negative rate of TRUST were 39.02% ,3.03% , and 60.98% respectively. The sensitivity, false positive rate and false negative rate of ELISA were 98, 73% ,20, 59% and 1.22%, Conclusion The combination of TRUST and ELISA is the first choice to screen patients with syphilis from blood donors. Moreover this screening contributes to blood safety.
出处
《实用医技杂志》
2007年第27期3709-3711,共3页
Journal of Practical Medical Techniques
