摘要
利用光合细菌Rhodopseudomonas 8株菌株研究初期活性、光照强度、C源种类、菌株差异对生物产氢的影响表明 ,不同菌株的C源利用性有较大差异 ,但对乳酸钠都有很好的利用性 .细菌的初期活性对产氢有一定程度的影响 ,稳定生长期的细菌比对数生长期的细菌产氢活性略高 .光照强度对产氢活性的影响明显 ,在光饱和度以下 ,光照强度大则产氢速率高 .不同菌种的产氢性能有效大差异 ,从上海地区有机污染环境中分离到的RhodopseudomonasB2 1 菌株在以乳酸钠 ( 50mmol·L- 1 )为C源、谷氨酸钠 ( 1 0mol·L- 1 )为N源 ,60 0 0Lx光照、30℃下 ,最大产氢速率达到 1 4.7ml·h- 1 ·g- 1 细胞干重 .
strains of photosynthetic bacteria Rhodopseudomonas were used to study the effect of bacterial initial activity, illumination intensity, carbon sources, strain differences on their hydrogen production. The results show that different strains are differed significantly in carbon source utilization,but they can utilize sodium lactate very well. Bacteria initial activity affects hydrogen production to a certain extent,and the hydrogen production activity of the bacteria is slightly higher in its stable growth phase than that in exponential growth phase.Illumination intensity has a significant influence on hydrogen production. Higher illumination intensity leads to higher hydrogen production rate.Strain B 21 of Rhodopseudomonas isolated from organically polluted areas of Shanghai has a very high hydrogen production rate, being 14.7 ml·h -1 ·g -1 (dry cell) with the carbon source of sodium lactate (50 mmol·L -1 ), nitrogen source of sodium glutamate (10 mmol·L -1 ), illumination intensity of 6000 lux, and the temperature of 30℃.
出处
《应用生态学报》
CAS
CSCD
1997年第2期194-198,共5页
Chinese Journal of Applied Ecology
基金
上海市环保局重点科研基金
关键词
光合细菌
产氢
影响因子
Photosynthetic bacteria, Hydrogen production, Affecting factor.
