摘要
参考genebank已发表的IBV纤突蛋白S2基因序列,设计了两对特异性引物,对鸡传染性支气管炎病毒黑龙江肾型分离株Mg/Mk和K进行RT-PCR扩增,将扩增后得到的PCR产物克隆入pMD18-T载体,并进行测序和分析。结果显示:S2基因的核苷酸全长为1884bp,编码一条由628个氨基酸组成的多肽,与网上报道的相一致。地方分离株Mg株、Mk株、K株分别和标准株T株比较,其同源性分别为92.3%、94.7%和94.2%。而Mg和Mk株的同源性为97.2%,Mg和K株的同源性为96.7%,Mk和K株的同源性为99.5%。说明地方分离株的亲缘关系较近,而和T株的亲缘关系较远。
According to the reported S2 spike protein gene sequence of IBV in genbank, two pairs of special primers were designed. After amplifying viral RNA of Mg/Mk/K/T strains by reverse transcription-polymerase chain reaction（RT-PCR） and cloning into pMD18-T vector, the products were sequenced and analyzed, Sequence analysis showed that full-length of S2 gene was 1884 bp and the S2 protein was composed of 628 amino acid residues after the gene was translated into amino acids. All the results suggested that they were the S2 gene of IBV. Comparative analysis of the homogeneity of Mg/ Mk/K and T were 92.3%, 94.7% and 94.2%. But homogeneity of Mg and Mk was 97.2%, homogeneity of Mg and K was 96.7%, homogeneity of Mk and K was 99.5%. This suggested that the relation of Mg, Mk and K is very near. The relation of Mg/Mk/K and T strain is far.
出处
《黑龙江八一农垦大学学报》
2007年第4期72-75,共4页
journal of heilongjiang bayi agricultural university
基金
黑龙江省骨干教师资助项目
农垦系统"九五"农业部重点科研项目(垦-06-25)。
关键词
S2基因
克隆
序列分析
S2 gene
Cloning
Sequencing analysis
