摘要
用随机扩增多态型ＤＮＡ（ＲＡＰＤ）技术，在幼苗期鉴定了圆叶葡萄和真葡萄亚属杂交的杂种。结果表明，以两个杂交组合后代及其亲本ＤＮＡ作模板，用随机引物ＵＢＣ－２５０，ＵＢＣ－２６６和ＵＢＣ－２７２可以分别扩增出１４００，１４４０，３５０和５００ｂｐ的多态性片段。
The identification of hybrids derived from Vitis rotundifolia×Vitis vinifera cultivar was carried out using RAPD techniques during seedling stage. The results showed the DNA polymorphisms of 1400,1440,350 and 500 bp were amplified by random primers of UBC 250,UBC 266,and UBC 272 and targeted to the molecular markers from seedless parents when the hybrids DNA were used as the templates from two cross combinations.Therefore,the hybrids,of both true and false would be determined by these molecular genetic markers before fruit production.
出处
《西北农业大学学报》
CSCD
北大核心
1997年第3期16-20,共5页
Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)
关键词
RAPD
圆叶葡萄
葡萄
杂种
分子标记
RAPD, Vitis rotundifolia,Vitis hybrid,molecular genetic marker
