摘要
应用基因工程技术对微生物细胞内的代谢通量进行重新设计,是获得高产高效的工业菌株的重要手段之一。基因敲除是20世纪80年代发展起来的一项重要的分子生物学技术,利用基因敲除技术可阻断细胞的代谢旁路,或通过引入突变位点改变目的产物的产量或质量而达到调节代谢流,优化代谢途径的目的。简单介绍了基因敲除技术的操作过程,重点讨论基因敲除技术的敲除策略及在微生物代谢工程方面的应用,并展望了相关技术在诸多领域的发展趋势。
Application of genetic engineering technology in the redesigning of the metabolic flux is one of the most important platform technology for strain improvement. A homologous recombination based, highly efficient molecular biology technology termed ‘ gene knockout’ has been developed since 1980s. The technology can regulate metabolic flux by inserting, deleting, cloning or substituting genomic DNA sequences at any desired position. The principles and operation proeedure of gene knockout were briefly introduced ,and its applications in metabolic engineering were discussed.
出处
《生物加工过程》
CAS
CSCD
2007年第3期10-14,共5页
Chinese Journal of Bioprocess Engineering
基金
国家自然科学基金资助项目(20336010)
关键词
基因敲除
代谢工程
同源重组
gene knockout
metabolic engineering
homologous recombination
