摘要
利用氧化乐果无机盐培养基通过长期的摇床驯化培养,从污染土壤中筛选出了可以在高浓度氧化乐果环境下生长的真菌菌株,并通过固体培养实验和液体培养实验以及有机磷显色反应最终筛选出两种生长较好的菌株,初步鉴定为曲霉。进一步研究发现,在50mL氧化乐果马铃薯液体培养基中,初始pH5.5,培养温度30℃,摇床转速150r·min-1,氧化乐果初始浓度2000μg·mL-1,培养时间10d,测得氧化乐果的降解率分别为70.38%和61.28%。研究了外界因素初始pH、培养温度、溶氧对降解菌的生长量和降解能力的影响,结果发现在培养5d,氧化乐果初始浓度2000μg·mL-1,初始pH5～7,培养温度30℃,摇床转速150r·min-1条件下,两降解菌均可达到最佳生长量和降解能力,生长量和降解能力具有正比例相关性变化趋势。
Strains which could grow in environment with high concentration of omethoate were screened from contaminated soil, incubated in omethoate and inorganic salt culture medium by long-term shaking. Ultimately, 2 strains were isolated by the solid culture experiment, liquid culture experiment and the color reaction of organophosphate. And they were identified as Aspergillus. Through quantitative biodegradation experiment, at initial pH 5.5, 30 ℃, shaking speed 150 r·min^-1, initial omethoate concentration 2 000 μg·mL^-1, culture time 10 d, the biodegradation rates of omethoate by the two strains were 70.38% and 61.28%, respectively. The effects of initial pH, culture temperature, dissolving oxygen on growth and omethoate-degradation of strains were studied. Under the condition of culture time 5 days, initial omethoate concentration 2 000μg·mL^-1, initial pH 5-7, temperature 30 ℃, shaking speed 150 r·min^-1, the two strains could grow best and have the highest biodegradation ability. And the growth of the strains related positively to the biodegradation rate of omethoate.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2007年第5期1744-1748,共5页
Journal of Agro-Environment Science
基金
安徽省科技厅重点计划项目(05023076)
关键词
氧化乐果
曲霉
土壤
omethoate
aspergillus
soil
