摘要
本研究检测β-catenin在白血病/淋巴瘤细胞系中的表达,探讨其与白血病的关系。采用半定量RT-PCR、免疫细胞化学和Western blot的方法检测β-catenin在白血病细胞系中的表达。结果表明:白血病细胞系中β-catenin在转录水平上有广泛表达,其中:在U937、KG1a、Jurkat、K562、Namalwa细胞中存在极高表达;在HEL、HUT78、Raji、Daudi、CEM中有中等表达;而在LCL-H、HL-60、NB4、J6-1、Ramos细胞中β-cateninmRNA呈相对较低水平的表达。蛋白表达水平与mRNA表达水平结果一致。所检测的细胞系中,胞核中均可见β-catenin的表达,但程度不一;在U937、K562、KG1a和Jurkat细胞的胞核内可见大量分布。结论:β-catenin作为WNT/β-catenin信号转导途径中重要的"调节子",其在白血病细胞中的过量表达提示WNT/β-catenin信号转导途径可能在白血病细胞中有异常激活。
This study was aimed to investigate the expression of β-catenin in leukemic cell lines and its relationship with pathogenesis of leukemia, semi-quantitative RT-PCR and Western blot were performed to detect the expression of β-catenin in a panel of 15 human hematopoietic cell lines （U937, KGla, Jurkat, K562, Namalwa, HEL, HUT78, Raji, Daudi, CEM, LCL-H, HL-60, NB4, J6-1, Ramos）. Immunocytochemistry was performed in some of these cell lines to detect the location of β-catenin. The results showed that the β-catenin gene was widely expressed in most leukemic cell lines in various degree, the high expression of β-catenin was found is U937, KGla, Jurkat, K562 and Namalwa cells, middle erpression of β-catenin was observed in HEL, HLT78, Raji, Daudi and CEM cells, lower expression of β-catenin was observed in LCL-H, HL-60, NB4, J6-1 and Ramos cells. The expression level of β-catenin protein was identical to the expression level of β-catenin mRNA. The expression of β-catenin could be found in nuclei of all cells mentioned above, but their levels were different belween them. Abundant β-catenin also could be observed in nuclei of some leukemic cells by immunocytochemistry. It is concluded that overexpression of β-catenin in leukemia cells, as a key mediator of Wnt signaling transduction pathway, indicates that the Wnt signaling transduction pathway may be aberrantly activated in leukemia.
出处
《中国实验血液学杂志》
CAS
CSCD
2007年第5期919-922,共4页
Journal of Experimental Hematology
基金
天津市自然科学基金资助项目(编号06YFJMSC08500)
人事部留学回国人员科技活动择优资助项目
