摘要
背景与目的:探讨五氯酚钠诱发中国仓鼠卵巢细胞(CHO)DNA损伤和染色体畸变的效应。材料与方法:五氯酚钠对中国仓鼠卵巢细胞(CHO)进行梯度染毒,应用单细胞凝胶电泳试验和体外染色体畸变试验观察其诱发细胞DNA损伤和染色体畸变。结果:80μg/ml和160μg/ml五氯酚钠诱发细胞DNA链断裂损伤的频率与对照组相比显著升高(P<0.01),且随着剂量增加,其损伤程度加重;无论是否加入代谢活化剂,受试物均引起染色体畸变率的明显增加(P<0.01)。结论:五氯酚钠能诱发CHO细胞DNA损伤和染色体畸变。
BACKGROUND ＆ AIM： To investigate the in vitro effects of DNA damage and chromosome aberration induced by sodium pentachlorophenol. MATERIALS AND METHODS： Different concentrations of sodium pentachlorophenol were added to the culture medium for ceils of hamster ovary （CHO） . DNA damage and chromosome aberration thus induced were evaluated by using single ceil gel electrophoresis（SCGE, comet assay） and chromosome aberration test. RESULTS： The frequencies of DNA damage caused by sodium pentachlorophenol in the treatment groups with dosages of 80 and 160 μg/ml were significantly higher than the control group, and showed a dose-response relationship. Sodium pentachlorophenol also induced a significantly increased chromosome aberration rate both with and without adding S9 mix. CONCLUSION： Sodium pentachlorophenol could induce DNA damage and chromosome aberration of CHO cells.
出处
《癌变．畸变．突变》
CAS
CSCD
2007年第5期355-357,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金重点项目(40332023)
关键词
五氯酚钠
单细胞凝胶电泳试验
体外
染色体畸变
DNA损伤
pentachlorophenol
single cell gel electrophoresis
in vitro
chromosome aberration
DNA damage
