摘要
目的：进一步阐明免疫紊乱在严重型再生障碍性贫血（ＳＡＡ）发病中的作用。方法：对１３例初诊ＳＡＡ、７例抗淋巴细胞球蛋白（ＡＬＧ）治疗后恢复期ＳＡＡ（ｒＳＡＡ）患者骨髓和外周血及对照组（包括９名骨髓对照和１１名外周血对照）的ＨＬＡ－ＤＲ＋Ｔ细胞进行检测，并对部分患者和对照组刺激的外周血去单核细胞的单个核细胞培养上清液（ＰＨＡ－ＬＹＣＭｓ）的造血活性进行检测。结果：初诊ＳＡＡ患者骨髓及外周血ＨＬＡ－ＤＲ＋Ｔ细胞显著高于对照组（Ｐ＜０．００１）；ＡＬＧ治疗后ｒＳＡＡ患者骨髓和外周血ＨＬＡ－ＤＲ＋Ｔ细胞较初诊ＳＡＡ下降，但仍高于对照组（Ｐ＜０．００１）；ＳＡＡ患者骨髓内ＨＬＡ－ＤＲ＋Ｔ细胞显著高于外周血（Ｐ＜０．００１）；与对照组比较，初诊的ＳＡＡ患者外周血ＰＨＡ－ＬＹＣＭｓ对正常骨髓ＢＦＵ－Ｅ和ＣＦＵ－ＧＭ具有显著的抑制作用（Ｐ＜０．００１），而ＡＬＧ治疗后ｒＳＡＡ患者ＰＨＡ－ＬＹＣＭｓ对正常骨髓ＢＦＵ－Ｅ和ＣＦＵ－ＧＭ的抑制作用明显减弱。结论：ＨＬＡ－ＤＲ＋Ｔ细胞在ＳＡＡ的发病中可能发挥重要的作用。
Ojective: To elucidate the role of immunodysfunction in the pathogenesis of severe aplastic anemia(SAA) . Methods: HLA DR + T cells were detected in bone marrow (BM) and peripheral blood(PB) of 20 SAA patients and controls (9 for BM and 11 for PB).PHA LYCM conditioned medium was prepared,and its in vitro effect on normal bone marrow BFU E and CFU GM was studied. Results: The percentages of HLA DR +T lymphocytes in BM and PB of newly diagnosed SAA patients were significantly higher than that of controls (P<0.001),and that of recovered SAA (rSAA) patients after ALG therapy(P<0.05).Compared with controls,the PBA LYCMs from newly diagnosed SAA patients showed significant inhibitory effect on normal BFU E and CFU GM (P<0.001). Conclusion: The activated T lymphocyte may play an important role in the pathogenesis of SAA.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1997年第4期186-189,共4页
Chinese Journal of Hematology
关键词
再生障碍性贫血
T细胞
造血抑制
骨髓
外周血
Anemia
aplastic
Activated T lymphocytes
Hematopoietic inhibitory effect
Bone marrow
Peripheral blood
