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应用酵母双杂交筛选与CUEDC2相互作用的蛋白质 被引量：1

Screening of Proteins Interacting with CUEDC2 by Yeast Two Hybrid
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摘要 目的:应用酵母双杂交技术,筛选与包含CUE结构域的人CUEDC2发生相互作用的蛋白质。方法:应用酵母双杂交系统,以CUEDC2为诱饵筛选人乳腺cDNA文库,寻找能与之相互作用的蛋白质,并运用营养缺陷型培养和X-alpha-Gal等实验提供的信息,筛除假阳性克隆。结果:以CUEDC2为诱饵最终筛出了2个阳性克隆,经测序及生物信息学分析,这2个克隆同为GADD34。结论:CUEDC2可以和GADD34发生相互作用,它们的相互作用有可能与CUEDC2的功能调控相关。 Objective： To find proteins interacting with human CUEDC2 by using yeast two-hybrid. Methods： Yeast twohybrid was applied to screen human breast cDNA library, and CUEDC2 as the baits respectively. Results： GADD34, growth arrest and DNA damage-inducible gene 34, was identified to interact with CUEDC2. Conclusion： GADD34 can interact with CUEDC2. Their interaction may suggest a new mechanism to regulate the function of CUEDC2.
作者 刘卉 潘欣 李慧艳 张佩景 李卫华 张学敏 周涛
机构地区 军事医学科学院仪器测试分析中心
出处 《生物技术通讯》 CAS 2007年第5期764-766,共3页 Letters in Biotechnology
基金 国家自然科学基金项目(30500583)
关键词 酵母双杂交 CUEDC2 GADD34 相互作用 yeast two hybrid CUEDC2 GADD34 interaction
分类号 Q25 [生物学—细胞生物学] Q78 [生物学—分子生物学]
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参考文献10

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同被引文献4

  • 1Feske, S, et al. A mutation in Orail causes immune deficiency by abrogating CRAC channel function [J]. Nature, 2006, 441 (7090) : 179 - 85.
  • 2Prakriya, M, et al. Orail is an essential pore subunit of the CRAC channel [J]. Nature, 2006, 443 (7108): 230-3.
  • 3Vig, M. et ol. CRACM1 multimers form the ionselective pore of the CRAC channel [J]. Curt Biol, 2006, 16 (20) : 2073 - 9.
  • 4Yeromin, A. V. et al. Molecular identification of the CRAC channel by altered ion selectivity in a mutant of Orai [ J ]. Nature, 2006. 443 (7108): 226-9.

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生物技术通讯
2007年 第5期

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