摘要
目的评价结核分枝杆菌蛋白芯片在检测临床标本中结核分枝杆菌抗体的应用价值。方法应用结核分枝杆菌蛋白芯片检测117例结核病患者的血清标本、103例肺部其他疾病患者和健康人的血清标本,并与痰涂片镜检法相比较。结果在菌阳肺结核、菌阴肺结核中,结核分枝杆菌抗体芯片检测的阳性率分别是100%(26/26)、49.6%(45/91),在肺部其他疾病患者和健康人中,结核分枝杆菌抗体芯片检测的阴性率是98.1%(101/103)。结论结核分枝杆菌抗体检测蛋白芯片是一种集基因工程技术、芯片技术和免疫学技术的检测一体化的新型结核病快速诊断方法,具有简便、快速、大量、敏感性高和特异性强、检测成本较低的特点,是结核病、尤其是菌阴结核病辅助诊断的有效方法。
Objective To evaluate the application value of protein-chip in detecting anti-tuberculosis （TB） antibodies in the clinical samples. Methods The serum samples from 117 patients with TB, and 103 patients with non-tuberculosis lung diseases and healthy volunteers were detected anti-TB antibodies using protein-chip. The sputum samples from 117 patients with TB were also observed acid-fast bacilli by smear and microscopy as control. Results The positive rate of anti-TB antibodies were 100% （26/26）, 49.6% （45/91） in smear-positive and smear-negative patients with pulmonary tuberculosis by using protein chip, respectively. The negative rate of anti-TB antibodies was 98.1% （101/103） in the patients with non-tuberculosis lung diseases and healthy volunteers by detection of protein chip. Conclusion The anti-TB antibody protein-chip is a simple, rapid, high-through, sensitive, specific, low cost, and effective method for the diagnosis of TB, especially for the smear-negative TB.
出处
《中国防痨杂志》
CAS
2007年第5期379-381,共3页
Chinese Journal of Antituberculosis
关键词
结核
肺/诊断
痰
蛋白芯片
Pulmonary tuberculosis/diagnosis
Sputum
Protein-chip
