摘要
目的探讨多种营养素联合拮抗铅对海马一氧化氮合成酶(NOS)活性和一氧化氮(NO)的作用,并寻找最佳具有拮抗作用的营养素组合。方法采用正交设计以染铅大鼠海马NOS和NO为检测指标,对酪氨酸、甘氨酸、蛋氨酸、牛磺酸、维生素C、维生素B1和锌等7种已知对铅毒性有拮抗作用和改善学习记忆功能的营养素进行筛选、配伍。结果给予营养素各组的血铅明显低于不给营养素的对照组(P<0.01),给予营养素各组的海马NOS活性和NO含量明显高于不给营养素的对照组(P<0.01)。正交实验设计结果为蛋氨酸、锌和维生素B1降低血铅作用明显,给与不给的血铅值极差差异有统计学意义;锌增强大鼠海马NO含量作用明显,其极差差异有统计学意义(P<0.01);维生素B1不具有增强大鼠海马NOS活性和增加大鼠海马NO含量作用,给予维生素的各组均值小于不给予各组,其极差差异有统计学意义(P<0.05)。结论多种营养素联合可改善铅对海马NOS和NO的作用,蛋氨酸、锌可降低血铅,提高海马中NOS活性,增加海马中NO含量的较好组合。
Objective To explore the combinative improvement effects of multi-nutrients on NOS, NO in hippocampus of SD rats exposed lead, and find the better combination of nutrients with antagonism to toxicity of lead. Methods Applying orthogonal design, taking the NOS, NO in hippocampus of rats as indices, 7 nutrients were tested on animal experiments. The nutrients were tyrosine, glycine, methionine, taurine, vitamin C, vitamin B1 and zinc, which were known to have the effects against toxicity of lead and improve learning and memory. Results The blood level of lead, activity of NOS and content of NO in hippocampus in the experimental groups supplied nutrients were greater than in control group exposed lead without nutrients （P 〈 0.01 ）. The results of orthogonal design showed that methionine, zinc and vitamin B1 decreased lead level in blood more significantly than other. There were statistic significance of mean difference between groups with those nutrients and without. Zinc increased the content of NO more obviously than other nutrients. Vitamin B1 couldn＇ t improve the activity of NOS, increase the content of NO. Mean of groups with vitamin B1 was smaller than one of groups without. There were significance of mean difference. Conclusion There exists synergic effect of mutil - nutrients on improving the activity of NOS, increasing the content of NO in hippocampus of rats exposed lead. The better combination of nutrients is methionine with zinc.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2007年第10期1180-1182,共3页
Chinese Journal of Public Health
基金
江西省自然科学基金(0540049)
江西省教育厅科技项目资助〔赣教技字(2007)110号〕
