摘要
目的研究不同剂量硒对染铅孕鼠胎盘功能和形态的影响。方法SD大鼠随机分为5组,自受孕1d～20d,中毒组孕鼠自由饮用含2mg/ml,醋酸铅的双蒸水,硒拮抗组在此基础上分别自由饮用含2,4,8μg/ml亚硒酸钠的双蒸水,对照组自由饮用双蒸水。孕20d时取材,应用原子吸收法测定血铅血硒;亚硝酸盐缓冲液法和免疫组化法分别测定血清碱性磷酸酶(ALP)活力和胎盘ALP含量,并在电镜下观察胎盘超微结构。结果4μgl/ml硒拮抗组(MA组)血铅和碱性磷酸酶水平与对照组比较差异无统计学意义(P<0.05),该组镜下超微结构与对照组比较无明显改变。各组血硒水平无明显变化。结论4μg/ml亚硒酸钠可明显拮抗2μg/ml醋酸铅对胎盘引起的毒性损伤,孕期适宜的硒营养补充量可明显改善铅暴露孕鼠胎盘功能。
Objective To avert the effect of various doses of selenium on lead - induced placenta of pregnant rats. Methods SD rats were randomly divided into 5 groups. From GD1 to GD20, the lead-poisoning group rats were fed 2mg/ml lead acetate solution by drinking. The selenium-rivalry group rats were fed 2, 4, 8μg/ml sodium-selenite solution by drinking on the base. The control group rats drink double evaporate water randomly. In GD250, the blood lead and selenium of pregnant rats were detected by atom absorption spectrum, and the serum alkaline phosphates by AMP balanced solution. The alkaline phosphates of placenta were detected by immunohistochemisty technique. The morphological changes of placenta were observed by the transmission election microscope and microscope. Results The level of blood lead and ALP had no singificant difference between MA group and the control group was very similar. There was no significant difference in the content of the blood selenium among all groups （ P 〈 0.05 ）. Conclusion 4μ/ml sodium-selenite can provide significant protective effects on the hazards of the 2 mg/ml lead acetate .in SD pregnant rats placenta. The selenium＇s eligible addition in pregnancy can improve the placental function obviously.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2007年第10期1229-1231,共3页
Chinese Journal of Public Health
