摘要
为探讨ＨＢＶＳ基因位点变异与血清学阴性乙型肝炎（乙肝）的关系，对长春、大同、杭州、深圳、青岛、西安、昆明和拉萨等８个城市，共计４３例血清学阳性和５２例血清学阴性乙肝病人血清ＨＢＶＳ基因第５２～５１２位核苷酸进行了序列测定和对比分析。发现同一基因型的ＨＢＶＳ基因序列较为保守，突变多呈随机性，未发现插入、缺失或无义突变；两类乙肝基因型和血清型均相同的Ｃ基因型／ａｄｒ血清型和Ｃ基因型／ａｄｗ２血清型，其ＨＢＶ分别有３个和５个高变异位点，Ｄ基因型／ａｙｗ１血清型（５株，均为血清学阳性乙肝ＨＢＶ）和Ｄ基因型／ａｙｗ３血清型（１０株，均为血清学阴性乙肝ＨＢＶ）有５个高变异位点，主要发生在血清学阴性乙肝组。上述结果表明，可能多数血清学阴性乙肝与血液中ＨＢＶ水平低有关，而少数由于ＨＢＶＳ基因变异所致。
Sequencing and comparative analysis of 52nd to 512th nucleotide of S gene in hepatitis B virus (HBV) were conducted in 43 cases of sero positive and 52 cases of sero negative HB from eight cities, including Changchun, Datong, Hangzhou, Shenzhen, Qingtao, Xi′an, Kunming and Lahsa, to study the relationship between focal variation of HBV S gene and seronegative HB. It was found that sequences of HBV S gene fragment in the same genotype were more conservative, most of its mutation happened randomly, and no insertion, deletion or null mutation occurred. There were three and five hypervariable nucleotide points in HBV isolated from two different HB groups with identical genotype and serotype (C genotype/adr serotype and C genotype/ayw1 serotype), respectively, and there were five hypervariable nucleotide points in HBV of D genotype/ayw1 serotype (five strains of sero positive HBV) and D genotype/ayw3 serotype (ten strains of sero negative HBV), respectively. It suggests that most cases of sero negative HB may correlate with low level of HBV in their blood, and a small number of cases may be caused by HBV S gene mutation.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
1997年第3期140-143,共4页
Chinese Journal of Preventive Medicine
