摘要
目的探讨急性冠脉综合征(ACS)患者树突状细胞(DC)功能状态以及依那普利(Enapril)对DC功能的影响。方法将42例ACS随机分为常规治疗组(21例)和常规治疗加依那普利治疗组(21例),分别于治疗前及治疗后1个月取血分离外周血T淋巴细胞,用流式细胞仪检测各组DC细胞表面共刺激分子CD86表达(以CD8+6CD11c+细胞比例表示);混合淋巴细胞反应(MLR)检测DC细胞对正常献血者T淋巴细胞刺激作用;分析依那普利治疗后CD8+6CD1+1c细胞比例,MLR的变化及其与血清C-反应蛋白(CRP)的相关性。结果与正常人比较,ACS患者DC细胞中CD8+6CD1+1c细胞比例明显增加(P<0.001);对T淋巴细胞增殖刺激作用明显增强(P<0.001);与治疗前比较依那普利治疗后CD8+6CD1+1c细胞比例和MLR明显下降(P<0.01);CD8+6CD1+1c细胞比例、MLR均与CRP呈正相关。结论ACS患者DC细胞功能明显增强;依那普利可能通过抑制DC细胞功能,负向调节患者的特异性免疫而抑制斑块的炎症反应。
Objective To investigate the dendritic cell function of acute coronary syndrome （ACS） patients and the modulation of DC function by Enalapril. Methods DCs were separated from peripheral blood of 42 patients with ACS （in routine or routine plus Enalapril groups） and induced in vitro in the presence of GM-CSF and IL-4 ; Flow cytometry was used to measure the surface co-stimulatory factor CDs6 expression （CD86^＋CD11c^＋） on DCs;Stimulating effect of DCs was determined with MLR. Results The expression of CD86^＋CD11c^＋ on DCs was much higher in ACS patients （compared with health individuals,P 〈 0.001 ） ;The stimulating effect of DCs to allogeneic lymphocyte was also enhanced in ACS patients versus normal control （P 〈 0.001 ） ;After treatment with Enalapril ,both CD86^＋CD11c^＋ and MLR were return to near normal （ compared with routine group, P 〈 0.01 ） ; The expression of CD86^＋CD11c^＋ and MLR are positively correlated with serum CRP level. Conclusions （ 1 ） The DCs were activated in ACS patients. （2） Enalapril inhibits DC function and may thus downregulate the immune response and attenuate the inflammation reaction in the plaque.
出处
《临床内科杂志》
CAS
2007年第9期614-616,共3页
Journal of Clinical Internal Medicine
基金
湖北省卫生厅科研基金项目资助(NX200511)
