摘要
目的:探讨大葱油对胃癌细胞的影响以及诱导胃癌细胞凋亡的机制.方法:MTT法评价大葱油对胃癌细胞增殖的影响.荧光染色观察细胞形态学变化.流式细胞术和放免法检测细胞凋亡及细胞内游离Ca2+和环磷酸腺苷(cAMP)浓度.结果:随着大葱油浓度的增加,作用24h后,细胞增殖抑制率分别达到5.71%,36.33%,44.34%,54.52%和74.15%,IC50≈65μg/mL.荧光显微镜下观察结果显示,实验组出现细胞凋亡形态学变化.100μg/mL大葱油作用于胃癌细胞1~12h,细胞总凋亡率分别为50.16%,53.48%,77.84%,83.71%和41.34%;早期细胞凋亡率分别为50.15%,33.25%,11.69%,4.65%和0.84%.100μg/mL大葱油作用于胃癌细胞5min^12h,各相应时间点细胞内游离Ca2+含量依次为(71.00±6.08),(77.67±1.53),(79.67±1.53),(83.33±5.03),(76.00±6.06),(75.67±2.52),(72.33±4.24)和(65.00±2.00)nmol/L;细胞内cAMP浓度依次为(5.76±0.36),(18.51±2.06),(20.40±1.65),(16.09±1.46),(12.16±0.78),(9.88±0.53),(9.47±0.78)和(9.82±1.06)pmol/mg(P<0.05).结论:大葱油可抑制胃癌细胞增殖,并通过上调细胞内Ca2+和cAMP水平诱导其凋亡.
AIM： To investigate the effects of Allium fistulosum L. oil （ALFO） on gastric cancer cell line and the possible signal molecular mechanism underlying apoptotic process induced by AFLO. METHODS： Human gastric cancer MGC80-3 cells were cultured in vitro and treated by AFLO. Then its growth inhibitory rate was counted and IC50 was measured. Apoptotic process was investigated with fluorescence microscope. Intracellular-free calcium content at different times was determined by flow cytometry. Intracellular cyclic adenosine monophosphate （cAMP） levels were determined by radioimmunoassay （RIA）. RESULTS： With increasing the concentration of AFLO, cell growth inhibitory rates went up correspondingly 24 h after treatment with AFLO and reached 5.71% , 36.33% , 44.34% , 54.52% and 74.15% respectively. ICs0 ~65 μg/mL. Hoechst 33258 fluorescent staining showed that apoptosis appeared in some cells treated by AFLO （100 μg/mL）. When MGC80-3 cells were incubated in the presence of AFLO （ 100 μg/mL） from 1 to 12 h, the total apoptotic rates were 50. 16%, 53. 48%, 77. 84%, 83. 71% and 41.34%, respectively, and the early apoptotic rates were 50.15% , 33.25% , 11.69%, 4.65% and 0.84%, respectively. In MGC80-3 cells treated by AFLO （ 100 μg/mL） for 5 min to 12 h, free calcium contents were （71.00 ±6.08） , （77.67 ± 1.53）, （79.67 ±1.53）, （83.33 ±5.03）, （76.00 ±6.06）, （75.67 ± 2.52 ）, （ 72.33 ± 4.24） and （65.00 ± 2.00） nmol/L, respectively and cAMP concentrations were （5. 76 ± 0. 36）, （18.51 ±2.06）, （20.40 ± 1.65）, （ 16.09 ± 1.46）, （12.16 ± 0.78）,（9.88±0.53）,（9.47±0.78）,（9.82 ±1.06） pmol/ mg, respectively. CONCLUSION： AFLO can inhibit the growth of gastric cancer cell line MGC80-3 and induce the apoptosis through increasing intracellular Ca^2＋ and cAMP levels.
出处
《第四军医大学学报》
北大核心
2007年第19期1771-1774,共4页
Journal of the Fourth Military Medical University
关键词
大葱油
胃肿瘤
肿瘤细胞
培养的
凋亡
CA^2+
CAMP
Allium fistulosum L. oil
stomach neoplasms
tumor cells, cultured
apoptosis
Ca^2＋
cAMP
