摘要
目的:分析TGF-β1在原发性肝癌(primary hepatic carcinoma,PHC)、癌旁组织、术后血清中表达特征与术后复发转移,生存率及手术方式的相关关系,探索PHC预后的量化评估方法。方法:采用免疫组化,夹心酶联免疫吸附试验,检测31例手术治疗的PHC不同组织学类型和不同手术方式患者,癌及癌旁组织、术后血清中TGF-β1表达水平,选择同期4例继发性肝癌(secondary hepatic carcinoma,SHC)手术病例对照,对其表达差异性及相关性进行统计学分析。结果:PHC和SHC癌及癌旁组织、术后血清TGF-β1的表达差异具有显著性意义。不同组织学类型的PHC表达不均衡,癌旁组织、术后血清TGF-β1表达随肝癌分型与分期越差而增强;胆管细胞癌高于肝细胞肝癌;肿瘤复发转移者高于无转移者;规则性肝切除高于局限性肝切除。结论:TGF-β1在癌旁组织和术后血清的表达程度与PHC的组织学分型暨肿瘤的恶性程度呈正相关,提示高恶性肝癌由于早期的浸润性生长方式刺激或激活肝脏间质组织或细胞合成分泌TGF-β1致使其过表达。术后血清中TGF-β1的表达与手术方式相关,显示规则性肝切除对肝脏损伤程度与范围大,肝细胞和间质组织及细胞的再生活跃,TGF-β1的合成旺盛。TGF-β1在术后血清中持续过表达可能是导致PHC复发转移和术后免疫功能缺陷的重要因素之一。
To study the correlation of transforming growth factor-beta 1 expression in primary hepatic carcinoma （PHC） tissues, para-cancer tissues and postoperative serum with postoperative relapse, metastasis, postoperative survival rate and different surgical procedures. Methods： SELISA and immunohistochemistry were used to detect the expression of TGF-β in PHC tissues, para-cancer tissues and postoperative serum in 31 eases with different histological types and surgical procedures. Four secondary hepatic carcinoma （SHC） cases were also tested. Results： There was a significant differenee in TGF-131 expression between PHC and SHC. The expression level of TGF-131 was higher in para-cancer tissues and postoperative serum of patients with more aggressive or more advanced carcinomas. The expression level of TGF-β1 was higher in patients with cholangiocellular carcinoma than in patients with hepatocellular carcinoma. The expression level of TGF-β1 was higher in patients with metastases than in patients without metastases. The expression level of TGF-β1 was higher in patients with standard hepatectomy than in patients with local hepatectomy. Conclusion： There is a direct correlation between the expression level of TGF-β1 and histological type and malignant degree of PHC, suggesting that the invasive growth of hepatic carcinoma stimulates interstitial tissues or cells to synthesize and secrete TGF-β1. The expression of TGF-β1 in postoperative serum correlates with different surgical procedure. Standard hepatectomy causes more damage to the liver and the subsequent repopu-lation of hepatocytes and interstitial tissues or postoperative serum is one of the factors related tive immune defect. cells synthesize TGF-β1. A high level of TGF-β1 in to the recurrence and metastasis of PHC and postoperative immune defect.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2007年第19期1101-1104,共4页
Chinese Journal of Clinical Oncology
基金
天津市应用基础研究计划面上项目资助(编号:05YFJMJCO8100)
