摘要
目的:研究原因不明习惯性流产(URA)患者的TORCH活动性感染率、淋巴细胞亚群及Th1/Th2细胞激活状态,以探讨与URA有关的发病机制。方法:用流式细胞术计数外周血淋巴细胞及其亚群;流式细胞仪结合微珠捕获法(Cytometric Bead Array,CBA)测定血清中6种Th1/Th2源细胞因子水平,酶联免疫吸附法(ELISA)检测血清中的TORCH抗体IgM和IgG。结果:(1)URA组的HSVⅡ-IgG和CMV-IgG的阳性率(46.2%,59.2%)分别高于对照组的阳性率(24.0%和44.8%),差异具有显著性意义(P<0.05);URA组的IgM类抗体阳性率全部高于对照组,差异均具有极显著性意义(P<0.01)。(2)URA患者外周血T细胞数:(62.9±7.2)%,较对照组(67.9±8.4)%减少有显著性意义(P<0.05);NK细胞数(16.3±8.4),较对照组(12.4±3.1)%增加有显著性意义(P<0.05)。(3)URA患者IL-2(6.1±1.6)ng/L及IL-5(4.5±1.4)ng/L较对照组(5.0±1.2)ng/L及(2.7±0.6)ng/L升高有显著性意义(P<0.05)。结论:(1)URA患者的活动性TORCH感染率显著升高;(2)URA的发生与该类患者T细胞总数减少以及NK细胞数量增加存在一定关联;(3)URA患者的Th1、Th2亚群均似有激活。
Objective： To investigate the role of infection with Toxoplasma gondii, rubella vires, cytomegalovirus （CMV） and herpersimples virus （ TORCH ） ,and lymphocyte subsets and TH1/TH2 active status in unexplained recurrent abortion（URA）, and so as to probe into the principle of the diseases, nethotis： The number of immunity cells and the active subsets in whole blood were counted with flow cytomter （FCM）. Cytometric bead array （CBA） was used to determine serum levels of cytokines derived from Thl/ Th2 helper lymphocyte, and ELISA was used to determine antibody IgM and IgG against TORCH in serum. Results： （1） The positive rates of HSVⅡ-IgG and CMV-IgG in URA patients were 46. 2% and 59. 2% repectively, which were higher significantly than those in controls （24. 0% ,44. 8% ） （P 〈0. 05）. The positive rates of four kinds of IgM were all higher significantly than those in control group （P 〈0. 01 ） ; （2） Compared with control group（67.9 ±8.4,12.4±3.1）, URA patients＇ CD3^＋ cell （62.9 ±7.2）fell and CD16^＋ 56^＋ cell （ 16.3 ± 8.4） rose significantly （ P 〈 0.05） ; （3） In URA patients, serum levels of IL-2 （6.1 ± 1.6） ng/L and IL-5 （4.5 ± 1.4 ） ng/L were higher than those in controls （5.0 ± 1.2） ng/L, （2.7 ± 0.6） ng/L（ P 〈 0.05）. Conclusions： （1）The active infection rates of TORCH in URA patients are increased; （2） URA is connected with falling of total T cell numbers and rising of NK cell numbers; （3） Both subsets Thl and Th2 seem to be activated in URA patients.
出处
《贵阳医学院学报》
CAS
2007年第5期484-487,491,共5页
Journal of Guiyang Medical College
