摘要
[目的]建立一种快速简便的回收分子量﹤10000 Da低丰度蛋白质的电洗脱方法。[方法]采用改进后的聚丙烯酰胺凝胶电泳和电洗脱方法分离纯化小分子标志蛋白,利用SELDI技术检测回收后的标志蛋白。[结果]SELDI技术检测发现,改进后的电泳和电洗脱方法能有效分离并回收10000 Da以下的低丰度蛋白质。[结论]改进后的电洗脱方法,不仅快速简便,而且还有利于蛋白质的后续鉴定。
[Objective]To recover less abundant protein with the MWT less than 10 000 Da with an effective electroeluting method.[Methods]The improved SDS-PAGE and electroeluting methods were used to separate and purify the small molecular protein,and SELDI technology was used to detect the recovered protein.[Results]The results of SELDI showed that less abundant proteins with the MWT less than 10 000 Da was effectively separated and recovered by improved SDS-PAGE and electroeluting methods.[Conclusion]The improved electroeluting method is not only effective to recover the less abundant protein with the MWT less than 10 000 Da,but also favourable to identify these proteins.
出处
《现代预防医学》
CAS
北大核心
2007年第19期3625-3626,3630,共3页
Modern Preventive Medicine
基金
广西科学基金项目(桂科攻0322025-2桂科攻0428005-12)
