摘要
目的探讨乙酰肝素酶(HPA)和缺氧诱导因子-1α(HIF-1α)与食管鳞癌发生、发展的关系。方法应用原位杂交技术检测70例食管鳞癌组织、26例癌旁不典型增生组织和23例切缘正常组织中HPAmRNA表达,同时,应用免疫组化法检测HIF-1α蛋白在全部标本中的表达。结果HPA mRNA和HIF-1α的阳性表达率在正常食管组织、癌旁不典型增生组织和食管癌组织中依次增高(P<0.05);HPA mRNA和HIF-1α的表达与食管鳞癌侵袭深度、淋巴结转移及临床分期有关(P< 0.05),而与分化程度无关(P>0.05)。在食管鳞癌组织中,HPA mRNA的表达与HIF-1α的表达显著相关(P<0.01)。结论HPA mRNA和HIF-1α阳性表达与食管鳞癌的生长、侵袭及转移有关,两者在食管鳞癌的发生、发展过程中可能存在着一定的协同作用;检测HPA mRNA与HIF-1α表达有助于食管鳞癌生物学性能的判断。
Objective To investigate the expression of HPA and HIF-lot in human esophageal squamous cell carcinoma and their relationship with cancer development, invasion and metastasis. Methods The expression of HPA mRNA and HIF-1α protein in 23 mucosa specimens of incisal margin, 26 paratumorous dysplastic tissues and 70 esophageal squamous cell carcinoma specimens were detected by in situ hybridization assay and immunohistochemical staining, respectively. Results The positive expression of HPA mRNA and HIF-1α protein were significantly increased as the epithelial cells progressed into carcinoma （ P 〈 0. 05 ）. The expression of HPA mRNA and HIF-1α protein in the esophageal squamous cell carcinoma were significantly correlated with the invasion depth, lymph node metastasis and clinical staging （ P 〈 0. 05）, while it was not correlated with the differentiation of tumors （P 〉0.05）. There was a close correlation between the expression of HPA mRNA and HIF-1α protein in the carcinoma tissues （ P 〈 0. 01 ）. Conclusion The high expression of HPA mRNA and HIF-1α protein is correlated with carcinogenesis, progression, invasion and metastasis of esophageal squamous cell carcinoma. There may be a positive cooperation between two of them in the carcinogenesis and development of esophageal carcinoma. The determination of HPA mRNA and HIF-1α will be useful in predicting tumor biological behavior.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2007年第9期689-692,共4页
Chinese Journal of Oncology
关键词
食管肿瘤
鳞癌
乙酰肝素酶
缺氧诱导因子-1Α
侵袭
转移
Esophageal neopalsms
Squamous cell carcinoma
Heparanase
Hypoxia inducible factors-1α
Invasion
Metastasis
