摘要
目的检测Ⅰ、Ⅱ、Ⅲ类整合子在嗜麦芽寡养单胞菌临床分离株中存在情况,分析整合子对细菌耐药性的影响。方法应用多重PCR方法筛选嗜麦芽寡养单胞菌整合酶基因。结果Ⅰ类整合子的检出率为13.4%,其中3株菌同时带有Ⅰ类和Ⅱ类整合子,Ⅱ类整合子的阳性率为3.6%,未检测到Ⅲ类整合子。结论嗜麦芽寡养单胞菌中主要分布Ⅰ类整合子,首次发现嗜麦芽寡养单胞菌中含有Ⅱ类整合子,Ⅱ类整合酶基因可能增加细菌多药耐药的发生。
OBJECTIVE To investigate the distribution and characterization of the classes Ⅰ , Ⅱand Ⅲ integrons on Stenotrophomonas maltophilia and clarify their influence on the bacterial drug-resistance. METHODS A multi- PCR assay using specific primers of int1, int2 and int3 was constructed to screen classesⅠ , Ⅱand Ⅲ integrons. RESULTS Class Ⅰ integron was detected in 13. 4% of clinical isolates, 3 isolates harbored among classⅡ integrons. There was not been reported in abroad. CONCLUSIONS Classes Ⅰand Ⅱintegrons could play an important role in causing the antibiotic multidrug resistance.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2007年第9期1061-1063,共3页
Chinese Journal of Nosocomiology
基金
北京市科委重大培育专项(Z0005190041891)
