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神经细胞黏附分子在GDNF保护大鼠多巴胺能神经元中的作用 被引量:1

The study of NCAM in mediating the protective effect of GDNF on dopaminergic neurons
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摘要 目的研究神经细胞黏附分子(neural cell adhesion molecule,NCAM)在胶质细胞系源性神经营养因子(glial cell line-derived neurotrophic factor,GDNF)保护帕金森(Parkinson's disease,PD)模型大鼠受损多巴胺(dopamine,DA)能神经元中的作用。方法右侧纹状体内立体定位注射6-羟多巴胺(6-OHDA)制备早期PD模型,而后分为4组:对照组(同侧黑质内注射PBS)、NCAM组(同侧黑质内仅注射anti-NCAM抗体)、GDNF组(同侧黑质内注射GDNF)、NCAM阻断组(同侧黑质内注射anti-NCAM抗体30min后注射GDNF),采用免疫组织化学染色技术和免疫印迹技术,观察各组酪氨酸羟化酶(tyrosine hydroxylase,TH)的表达变化。结果GDNF组黑质致密部TH阳性神经元数目及表达的量明显多于PBS组,差别有统计学意义;NCAM阻断组与GDNF组相比,该处TH阳性神经元数目及表达的量明显减少,差别有统计学意义。结论NCAM参与了GDNF保护DA能神经元的作用。 Objective To explore whether neural cell adhesion molecule (NCAM) mediates the protective el-fect of glial cell line-derived neurotrophic factor(GDNF) on injured dopaminergic (DA) neurons. Methods The early period of Parkinson's disease(PD)models were established by stereotaxic injection of 6-OHDA. The rats were divided into 4 groups respectively :control group, NCAM group ,GDNF group ,and GDNF+anti-NCAM group. Immunohistochemistry and Western blot were used to examine the number of TH-positive cells and the expresse level of TH protein in SN of rat. Results GDNF significantly increased the number of TH-positive cells and the ex- pressing of TH as compared to control group. However,when the NCAM pathway was blocked by injecting anti NCAM,the number of TH positive cells and the level of TH were significantly decreased. Conclusion GDNF protects the injured DA neurons,and NCAM may be involved in protective effect of GDNF on DA neurons.
出处 《中风与神经疾病杂志》 CAS CSCD 北大核心 2007年第4期428-430,共3页 Journal of Apoplexy and Nervous Diseases
基金 国家自然科学基金资助项目(No.30570564)
关键词 胶质细胞系源性神经营养因子 神经细胞黏附分子 酪氨酸羟化酶 Glial cell line-derived neurotrophic factor Neural cell adhesion molecule Tyrosine hydroxylase
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参考文献7

  • 1Lin LF,Doherty DH,Lile JD,et al.GDNF:a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons[J].science,1993,260(5111):1130-1132.
  • 2姜晓兵,赵洪洋,周凤,刘如恩,周伟,张建国.稳定表达GDNF基因的骨髓基质干细胞对多巴胺能神经元的作用[J].中风与神经疾病杂志,2004,21(4):311-313. 被引量:1
  • 3Grondin R,Zhang ZM,Yi A,et al.Chronic,controlled GDNF infusion promotes structural and functional recovery in advanced parkinsonian monkeys[J].Brain,2002,125(10):2191-2201.
  • 4Treanor JJ,Goodman L,Saurage F,et al.Characterization of a multicomponent receptor for GDNF[J].Nature,1996,382(6586):80-83.
  • 5Paratcha G,Ledda F.The neural cell adhesion molecule NCAM is an alternative signaling receptor for GDNF family ligands[J].Cell,2003,113(7):867-879.
  • 6刘洪梅,王炎强,丁艳霞,王红军,高殿帅.早期帕金森病大鼠模型的建立[J].徐州医学院学报,2005,25(6):481-484. 被引量:10
  • 7Chao CC,Ma YL,Chu KY,et al.Integrin alpha V and NCAM mediate the effects of GDNF on DA neuron survival,outgrowth,DA turnover and motor activity in rats[J].Neurobiol Agging,2003,24(1):105-116.

二级参考文献15

  • 1Lin LF,Doherty DH,Lile JD,et al. GDNF:a glial cell line-derived neurotrophic factor form midbrain dopaminergic neurons [J]. Science, 1993,260(5111): 1130-1132.
  • 2Hou JG,Lin LF,Mytilineou C. Glial cell line-derived neurotrophic factor exerts neurotrophic effects on dopaminergic neurons in vitro and promotes their survival and regrowth after damage by 1-methyl-4-phenylpyridinium [J]. J Neurochem, 1996, 66 (1): 74-82
  • 3Williams LR,Inouye G,Cummins V,et al. Glial cell line-derived neurotrophic factor sustains axotomized basal forebrain cholinergic neurons in vivo: dose response comparison to nerve growth factor and brain-derived neurotrophic factor[J]. J Pharmacol Exp Th
  • 4Muraglia A ,Cancedda R,Quarto R. Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model[J]. J Cell Sci,2000,113(7): 1161-1162.
  • 5Brazelton TR, Rossi FM. From marrow to brain: expression of neuronal phenotypes in adult mice[J]. Science, 2000,290 (5497):1775-1779.
  • 6Sanchez R J, Song S, Cardozo PF,et al. Adult bone marrow stromal cells differentiate into neural cells in vitro[J]. Exp Neurol,2000,164 (2): 247-256.
  • 7Azizi SA,Stokes D, Augelli BJ,et al. Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rats-similarities to astrocyte grafts[J]. Proc Natl Acad Sci USA, 1998,95(7): 3908-3913.
  • 8Li Y,Chen J,Wang L,et al. Treatment of stroke in rat with intracarotid administration of marrow stromal cells[J]. Neurology,2001,56(12) :1666-1672.
  • 9Haynesworth SE,Baber MA,Caplan AI. Cytokine expression by human marrow-derived mesenchymal progenitor cells in vitro:effects of dexamethasone and IL-1 alpha[J]. J Cell Physiol,1996,166(3): 585-592.
  • 10Ho SH,So LH,Lai KO,et al. Induction of NG108-15 cell differentiation by human marrow stromal cells [J]. Neuroreport,1998,9(7): 1365-1369.

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