摘要
The dimerization of a well-known DNA light-switch [Ru(phen)2dppz]2+(phen=1,10-phenanthroline, dppz=dipyrido[3,2-a:2′,3′-c]phenazine), and its effect on calf thymus binding properties were studied by UV-Vis and emission spectroscopy in Tris-HCl buffered 50 mmol/L NaCl(pH=7.1). The results show that the complex has a strong tendency to dimerize with a dimerization constant of (1.8±0.3)×106 L/mol at the same order of magnitude as the DNA binding constant of (6.0±1.2)×106 L/mol for the Ru(Ⅱ) complex dimer, but one order of magnitude less than (4.9±1.5)×107 L/mol for the Ru(Ⅱ) complex monomer. This study has provided insight into a fundamental but important issue that should be taken into account for the DNA binding studies.
The dimerization of a well-known DNA light-switch [ Ru（phen） 2dppz]^2 ＋（ phen = 1,10-phenanthroline, dppz = dipyrido [ 3,2-a ： 2＇, 3＇-c] phenazine）, and its effect on calf thymus binding properties were studied by UV-Vis and emission spectroscopy in Tris-HCl buffered 50 mmol/L NaCI（pH = 7. 1 ）. The results show that the complex has a strong tendency to dimerize with a dimerization constant of （1.8±0.3 ） × 10^6 L/ mol at the same order of magnitude as the DNA binding constant of （6.0 ± 1.2） × 10^6 L/mol for the Ru（ Ⅱ ） complex dimer, but one order of magnitude less than （4. 9 ±1.5 ） × 10^7 L/mol for the Ru（ Ⅱ ） complex monomer. This study has provided insight into a fundamental but important issue that should be taken into account for the DNA binding studies.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2007年第10期1833-1835,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:20371008
90401007)
北京市自然科学基金(批准号:2052008
2072011)
高等学校博士学科点专项科研基金(批准号:20060027002)
北京师范大学本科生科学研究基金资助.
关键词
钌
邻菲啰啉
DNA
光开关
二聚
Ruthenium
Phenanthroline
DNA
Light switch
Dimerization
