血管生成素Ⅱ在低压性缺氧成年大鼠视网膜的表达被引量：6

Angiopoietin Ⅱ in retina of hypoxic adult rats

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摘要目的探讨成年大鼠缺氧后血管生成素Ⅱ（angiopoietinⅡ,AngⅡ）在视网膜中的表达情况。方法成年Wistar大鼠暴露于低氧环境（70~90mL.L-1O2）2h后,分别在3h、24h、3d、7d和14d,通过实时荧光定量逆转录聚合酶链反应、蛋白免疫印迹法检测AngⅡ在视网膜中的表达情况。结果缺氧后3h、24h和3d,成年大鼠视网膜AngⅡmRNA水平与正常对照组相比分别增加120%、50%、30%（P〈0.05）,AngⅡ蛋白质水平与正常对照组相比分别增加了90%、32%、20%,而在7d和14d时其表达较正常对照组差异无显著性。结论缺氧可以使成年大鼠视网膜AngⅡ表达上调。 Objective To investigate the expression of angiopoietin Ⅱ （Ang Ⅱ ） in the retina of adult rat following hypobaric hypoxic exposures. Methods Adult Wistar rats were exposed to hypobaric hypoxic environment for 2 hours following which the expression of Aug Ⅱ were observed at 3 hours, 24 hours, 3 days,7 days and 14 days with real time quantitative RT-PCR and Western-blotting,respectively. Results Compared with normal control group, the expression of Aug Ⅱ mRNA were increased by 120% ,50% ,30% and the level of proteins increased by 90% ,32% ,20% ,respectively, following hypobarie hypoxic exposures for 3 hours,24 hours and 3 days（P〈0.05）.There were no significant differences at 7 days and 14 days. Conclusion Hypoxia can upregulate Aug Ⅱ in adult retina

作者张勇王志斌周霞许玲芦晓磊黄剑云

机构地区郧阳医学院附属太和医院眼科

出处《眼科新进展》 CAS 2007年第10期758-761,共4页 Recent Advances in Ophthalmology

关键词血管生成素Ⅱ 缺氧视网膜实时荧光定量逆转录聚合酶链反应蛋白免疫印迹法 angiogenin Ⅱ hypoxia retina real time quantitative RT-PCR western-blotting

分类号 R774.2 [医药卫生—眼科]

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1Cohen LH, Noell WK.Glucose catabolism of rabbit retina before and after development of visual function [ J].J Neurochem 1960 ;5(5) :253-276.
2Anderson B Jr, Saltzman HA. Retinal oxygen utilization measured by hyperbaric blackout [ J]. Arch Ophthalmol 1954 ;12 (72):792-795.
3Ottino P, Finley J, Rojo E, Ottlecz A, Lambrou GN, Bazan HE,et al. Hypoxia activates matrix metalloproteinase expression and the VEGF system in monkey choroid-retinal endothelial cells: Involvement of cytosolic phospholipase A2 activity [J] .Mol Vis 2004;17(10) :341-350.
4Wise GN. Retinal neovascularization [ J]. Trans Am Ophthalmol Soc 1956;1 (54) :729-826.
5Lutty GA, McLeod DS. Retinal vascular development and oxygen-induced retinopathy:a role for adenosine [ J] .Prog Retin Eye Res 2003;22 ( 1 ) :95-111.
6Lobov IB, Brooks PC, Lang RA. Angiopoietin-2 displays VEGF-dependent modulation of capillary structure and endothelial cell survival in vivo[J]. Proc Natl Acad Sci USA 2002 ;99(17) :11205-11210.
7Livak K J, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PIER and the 2^-△△Ct method [J]. Methods 2001 ;25 (4) :402-408.
8Hacker SF, Ozaki H, Strauss RW, Wahlin K, Suri C, Maisonpierre P,et al. Angiopoietin 2 expression in the retina: upregulation during physiologic and pathologic neovascularization[ J] .J Cell Physiol 2000;184(3) :275-284.
9Hackett SF, Wiegand S,Yancopoulos G, Campochiaro PA.Angiopoietin-2 plays an important role in retinal angiogenesis [J]. J Cell Physiol 2002 ; 192 ( 2 ) : 182-187.
10Ray PS,Estrada-Hernandez T,Sasaki H,Zhu L,Maultk N. Early effects of hypoxia/reoxygenation on VEGF, ang-1, ang-2 and their receptors in the rat myocardium:implications for myocardial angiogenesis[J]. Mol Cell Biochem 2000;213( 1 ) :145-153.