摘要
目的建立可同时检测人血浆中替米沙坦、氢氯噻嗪含量的反相高效液相色谱方法。方法色谱条件:色谱柱为ZorbaxsoA Extend-C18(4.6mm×150mm,5μm);流动相为乙腈-四丁基氢氧化铵磷酸盐缓冲液(24:76);流速:1.0mL·min^-1;检测波长:230nm(替米沙坦),272nm(氢氯噻嗪);柱温:20℃。结果本法替米沙坦的线性范围为3l.8~l591.2μg·L^-1,r=0.9994.方法回收率为98.68%,RSD=3.09%,最低检测质量浓度为20μg·L^-1,氢氯噻嗪的线性范围为30~240μg·L^-1,r=0.9991.方法回收率为100.32%,RSD=5.11%,最低检测质量浓度为20μg·L^-1结论本法可以同时测定血浆中替米沙坦和氢氯噻嗪的浓度,具有样品处理简单、方法稳定、有较好的实用性,易于开展药动学及药效学研究。
OBJECTIVE To establish a method for simultaneous determination of telmisartan and hydrochlorothiazide in human plasma. METHODS The concentrations of telmisartan and HCTZ were analysed on a Zorbax 80A Extend -C18 (4.6 mm×150 mm,5 μm) column with a mobile phase consisting of tetrabutylammonium hydroxide phosphate buffer solution ( pH 2.26 ) -acetonitrile (76: 24 ) at a flow rate of 1 mL · min^-1, the wavelengthes were 230 and 272 nm, respectively. RESULTS The calibration curve of TMST was linear with the range of 31.8 - 1 591.2 μg · L^-1. The minimum eoncentration was 20 μg · L^-1. The calibration curve of hydrochlo- rothiazide was linear with the range of 30 - 240 μg · L^-1. The minimum concentration was 20 μg · L^-1. CONCLUSION It is a rapid, reproducible method. Clinical application demonstrated that this method was practical and it can be completely used in the study of pharmacokinetics and pharmacodynamics.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2007年第19期1496-1498,共3页
Chinese Pharmaceutical Journal