摘要
研究了氨苄青霉素与对苯醌的荷移反应，确定了反应条件。在ｐＨ ４．４～５．０的醋酸－醋酸钠缓冲溶液中形成１：１络合物，其λｍａｘ＝４７２ ｎｍ。氨苄青霉素三水合物浓度在 １０～１２５ｍｇ／Ｌ范围内呈线性关系；当浓度为 ５０ ｍｇ／Ｌ时相对标准偏差为 １．８％（ｎ＝１０）；表观摩尔吸光系数为２．６４ × １０３Ｌ·ｍｏｌ－１·ｃｍ－１。应用拟定的方法测定药物制剂含量，结果与药典方法一致。回收率在９６％以上。
A spectrophotometric method for the determination of ampicillin has been devel- oped based on the charge transfer reaction. Beer's law is obeyed in the range of 10-l25 mg/L of ampicillin trihydrate. The apparent molar absorptivity of complex at 472 nm is 2. 64 ×103L. mol-1cm-1. The average standard deviation of ten determination is l. 8%. The composition of ampicillin-p-benzoquinone complex is l: l. The proposed method has been applied to the determination of ampicillin in capsules and dry syrup with satisfactory results.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1997年第1期46-48,共3页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助课题.
关键词
荷移反应
氨苄青霉素
分光光度法
Charge transfer reaction, ampicillin, spectrophotometry
