摘要
目的检测宫颈癌细胞系HeLa、CaSki和SiHa中结肠腺瘤性息肉病基因(APC)启动子区CpG岛甲基化状态;探索肼苯哒嗪(Hyd)能否作为去甲基化药物恢复APC表达。方法应用甲基化特异性聚合酶链反应(MSP)检测细胞系中APC启动子区CpG岛甲基化情况;Hyd处理后,RT-PCR检测APCmRNA表达。结果APC启动子区CpG岛在HeLa细胞中被甲基化,在CaSki细胞中半甲基化,在SiHa细胞中无甲基化;一定浓度Hyd处理HeLa、CaSki细胞后,检测到APCmRNA表达。结论HeLa、CaSki细胞系APC表达沉默与APC启动子区甲基化有关;Hyd能作为去甲基化药物诱导APC表达。
Objective To detect 5＇CpG island methylation in cervical carcinoma cell lines （HeLa, CaSki, SiHa）, and of APC （ adenomatous polyposis coli ） gene promoter region to investigate the possibility of Hydralazine in restoring the expression of APC gene through demethylation. Methods The CpG island methylation status of APC gene promoter region of the cervical carcinoma cell lines were analyzed using methylated specific polymerase chain reaction （MSP）. The mRNA expression profile of APC gene was analyzed by using RT-PCR after Hydralazine treatment. Results The CpG island of APC gene was methylated in HeLa and hemimethylated in CaSki but not in SiHa. The mRNA expression of APC gene can be detected after hydralazine treatment. Conclusion The APC gene non-expression in cervical carcinoma is associated with CpG island methylation in APC gene promoter region. Hydralazine, served as a demethylating agent, enables to restore the expression of APC gene.
出处
《基础医学与临床》
CSCD
北大核心
2007年第9期981-984,共4页
Basic and Clinical Medicine
基金
湖北省卫生厅科研基金(JX2B76)
