期刊文献+

苦参碱对人单核细胞内三磷酸腺苷结合盒转运体A1及胆固醇酯的影响 被引量:1

Effects of matrine on ATP binding cassette transporter A1 and cholesterol in monocyte cells
下载PDF
导出
摘要 目的观察苦参碱对人单核细胞胆固醇和三磷酸腺苷结合盒转运体A1(ABCA1)表达的影响。方法用TBARS法检测细胞内MDA,油红O染色法检测泡沫细胞的形成,荧光分光光度法检测细胞内总胆固醇、游离胆固醇和胆固醇酯含量,RT-PCR和Western blot检测ABCA1mRNA与蛋白的表达。结果单核细胞经佛波脂(PMA)及ox-LDL共同孵育后,细胞内积聚的总胆固醇、游离胆固醇、胆固醇酯及脂质过氧化产物均明显增多(P<0.05),有大量泡沫细胞形成,而ABCA1蛋白、mRNA水平明显减少(P<0.05);苦参碱干预组显著缓解上述变化,细胞内胆固醇酯减少,ABCA1 mRNA、蛋白表达明显增加(P<0.05)。结论苦参碱可能通过增强ABCA1的表达和降低细胞内胆固醇聚积而发挥抗动脉粥样硬化的作用。 Objective To study the effect of matrine on ATP binding cassette transporter A1 (ABCA1) expression and cholesterol in monocyte derived foam cells. Methods The lipid peroxide in cells was measured by TBARS method; The foam cells were examined by oil red 0 stain. The accumulation of cholesterol in monocyte was measured by fluorescence spectrophotometric method;ABCA1 mRNA and its protein level were determined by RT-PCR and Western blot, respectively. Results In the homonocytes incubated with PMA and low density lipoprotein (LDL), the intracellular accumulated total cholesterol (TC), free cholesterol (FC), cholesteryl ester (CE) and lipid peroxide increased obviously, and a huge amount of foam cells were found by oil red O stain. This was accompanied by a reduction in the membrane content of ABCA1. matrine alter ABCA1 mRNA abundance, indicating that increased ABCA1 transcription, enhanced mRNA level. Conclusion The mechanism of anti-artherosclerosis by matrine may be related to reduce cholesterol and to increase the level of ABCA1 expression.
出处 《基础医学与临床》 CSCD 北大核心 2007年第9期996-1000,共5页 Basic and Clinical Medicine
基金 宁夏自然科学基金(NZ0534)
关键词 苦参碱 三磷酸腺苷结合盒转运体A1 胆固醇 氧化型低密度脂蛋白 matrine ATP binding cassette transporter A1 cholesterol low density lipoprotein
  • 相关文献

参考文献12

  • 1RigotV, HamonY, Chambenoit O, et al. Distinct sites on ABCA1 control distinct steps required for cellular release of phospholipids[ J]. J Lipid Res, 2002, 43 (12):2077- 2086.
  • 2Van Oostrom A J, van Wijk J, Cabezas MC. Lipaemia, inflammation and atherosclerosis: novel opportunities in the understanding and treatment of atherosclerosis [ J ]. Drugs, 2004, 64(12) :19 -22.
  • 3冯晓,汤焕梅,沈烈行.苦参碱的药理学研究概况[J].药物流行病学杂志,2005,14(6):331-334. 被引量:26
  • 4刘梅,刘雪英,程建峰.苦参碱的药理研究进展[J].中国中药杂志,2003,28(9):801-804. 被引量:216
  • 5Xiaokun Zeng, Jing Dai, Xian Wang, et al. Homocysteine mediated expression and secretion of monocyte chemoattractant protein-1 and interleukin-8 in human monocytes [ J ]. Circulat Res, 2003,93:311.
  • 6Wada Y, Sugiyama A, Yamamoto T, et al. Lipid accumulation in smooth muscle cells underLDL loading is independent of LDL receptor pathway and enhanced byhypoxic conditions [ J ]. Arterioscler Thromb Vasc Biol, 2002, 22 ( 10) : 1712 - 1719.
  • 7侯燕芝,陈瑞,陈安宇,于培兰,史小苓,孙林,王锐.微波辐照对小鼠脑内MDA、DA和NE含量及NOS活性的影响[J].基础医学与临床,2004,24(3):350-351. 被引量:2
  • 8OramJ F, Lawn RM. ABCA1 :the gatekeeper for eliminating excess tissue cholesterol [ J ]. Lipid Res, 2001,42 (8) : 1173 - 1179.
  • 9张文成,陈保生,曾武威,吴刚,薛红.低密度脂蛋白诱导下调的新基因cDNA的克隆及组织表达[J].基础医学与临床,2003,23(3):279-282. 被引量:22
  • 10Chambenoit O, Hamon Y, Marguet D, et al. Specific docking of apolipoprotein A-I at the cell surface requires a functional ABCA1 transporter [ J]. J Biol Chem, 2001, 276 ( 13 ) : 9955 - 9960.

二级参考文献85

共引文献278

同被引文献7

引证文献1

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部