摘要
目的了解吉林延边地区朝鲜族人群3个短串联重复序列（short tandem repeats，STR）基因座D4S2368、D6S1043、D9S925的遗传多态性分布，获取相应多态位点的群体遗传学数据。方法采用PCR扩增技术及聚丙烯酰胺凝胶电泳方法，对310名朝鲜族个体的3个STR基因座的等位基因频率进行分析。结果在3个基因座D4S2368、D6S1043、D9S925分别检出7、13、9个等位基因，除D6S1043（P=0．011）以外，其他两个基因座多态性分布均符合Hardy．Weinberg平衡定律（P〉0．05）。杂合度大于0．717，多态信息含量大于0．670，累积个人识别力及非父排除率分别为0．9995和0．952。结论上述3个基因座均具有较高的杂合度和多态信息量，是较理想的遗传标记系统，对朝鲜族群体的亲子鉴定、个人识别及民族基因库的建立具有重要意义。
Objective To understand the allele structure and genetic polymorphism at D4S2368, D6S1043, D9S925 short tandem repeat （SI＇R） loci in Korean ethnic group of Jilin, and to construct a preliminary database. Methods The allele frequencies of the three SIRs loci in 310 unrelated individuals from Korean ethnic individuals were analyzed by polymerase chain reaction （PCR） and polyacrylamide gel electrophoresis （PAGE）. Results Seven, thirteen, and nine alleles were observed at D4S2368, D6S1043, and D9S925 loci, respectively, and all loci met Hardy-Weinberg equilibrium （except D6S1043）. The statistical analysis of 3 STR loci showed the heterozygesities were more than 0.717, the polymorphic information contents （PIC） were more than 0.670; the combined power of discrimination （PD） and the power of exclusion （PE） were more than 0.9995 and 0.952 respectively. Conclusion The three loci in this study are found to have high heterozygosity and polymorphic information content, so they can provide useful markers for genetic purposes. These results could serve as valuable data to enrich the Korean ethnic group genetic database and play an impoaant role in Chinese population genetic application.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2007年第5期592-593,共2页
Chinese Journal of Medical Genetics
基金
教育部留学回国人员科研启动基金（2005-383）
关键词
朝鲜族
短串联重复序列
聚合酶链反应
遗传多态性
Korean ethnic group
short tandem repeat
polymerase chain reaction
genetic polymorphism
