摘要
目的建立甲磺酸帕珠沙星的血药浓度的高效液相色谱方法。方法以特拉唑嗪为内标,以ZORBAX C18（4.6mm×150mm,4.6μm）色谱柱为分析柱;采用乙腈-0.01mol.L-1柠檬酸水溶液-三乙胺-冰醋酸（13.9∶85.7∶0.3∶0.1,v/v/v/v,pH值5.5）为流动相;流速为1ml/min;激发波长为335nm;发射波长为450nm。结果甲磺酸帕珠沙星线性范围0.037 2-19.5333μg.ml-1,定量下限为0.0372μg.m^l-1。日内、日间精密度RSD〈8%,平均回收率〉50%。结论本方法简便、准确、灵敏,特异性强,重复性好,适用于甲磺酸帕珠沙星临床药动学研究。
Aim To establish an HPLC method for determination of Pazufloxacinmesilate in human blood serum. Methods Terazosin was used as the internal standard with the ZORBAX C18 （4.6mm × 150mm ,4.6μm） as the analytical column. The mobile phase consisted of acetonitrile-0.01 mol·L^- 1 citric acid-triethylamine-acetum （ 13.9： 85. 7： 0.3： 0.1, v/v/v/v, pH：5.5）. The flow rate was 1.0ml/min with fluorescence detection set at λex 335nm and λem 450nm. Results The linear range of the calibration curve was within drug sersum concentrations of 0.037 2 19.533 3μg·ml^-1. The detection limit was 0.037 2μg·ml^-1 （ n =5）. The average recovery was above 50%, The inter and intra-day precision （ RSD ）was less than 8%. Conclusions The method is shown to be sensitive, accurate, simple and reproducible for the determination of Pazufloxacinmesilate levels in human blood serum. It is suitable for pharmacokinetic study of Pazufloxacinmesilate.
出处
《解放军药学学报》
CAS
2007年第5期387-389,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
