穹窿海马伞切断和卵巢切除对大鼠不同脑区雌激素受体α与淀粉样β蛋白前体蛋白β位点裂解酶共存表达的影响被引量：1

Coexist expression and relation of estrogen receptor α and β-site β-amyloid precursor protein cleaving enzyme in rat′s brain with fimbria/fornix transection and ovariectomy

下载PDF

导出

摘要目的:探讨穹窿-海马伞切断和卵巢切除大鼠大脑雌激素受体α(ERα)与β淀粉样蛋白前体蛋白β位点裂解酶(BACE)共存表达的变化。方法:成年健康雌性Wistar大鼠28只,随机分为穹窿-海马伞切断组(FF组)、卵巢切除组(OVX组)、穹窿-海马伞切断加卵巢切除组(OF组)和对照组。每组7只。免疫荧光双标细胞化学法染色,激光共聚焦显微镜下观察大鼠大脑皮质区、海马CA1区ERα、BACE阳性细胞表达的变化。结果:FF组、OVX组和OF组大鼠皮质区和海马CA1区ERα阳性细胞与对照组比较显著减少(皮质区:4.71±1.11,5.29±2.01,4.42±3.99vs9.71±4.53;海马区:6.91±2.63,7.58±4.16,5.14±3.80vs12.57±3.59,均P<0.05);FF组、OVX组和OF组大鼠皮质区和海马CA1区BACE阳性细胞与对照组比较显著增多(皮质区:15.78±6.01,17.63±4.42,20.00±5.16vs5.71±3.14;海马区:15.91±5.12,12.34±5.07,17.14±4.45vs6.85±1.95,均P<0.05)。但三组之间两两比较无显著性差异(P>0.05)。OF组大鼠皮质区ERα和BACE双标阳性细胞数与对照组比较显著增多(P<0.05),但海马CA1区无显著性变化(P>0.05)。结论:穹窿-海马伞切断或卵巢切除可以导致大鼠大脑皮质区、海马CA1区ERα表达减少、BACE表达增多,两种方法同时作用导致皮质区共存细胞表达增多。 Objective： To observe the coexist expressive variety of estrogen receptor α（ERα） and β-site β-amyloid precursor protein cleaving enzyme （DACE） in the cerebral cortex and hippocampus CA1 of rat＇s brain with fimbria/ fornix transection and ovariectomy. Method：Twenty-eight healthy female rats were randomly divided into four groups, fimbria/fornix transection group（FF）, ovariectomized group（OVX）, fimbria/fornix transection and ovariectomized group（OF）, control group. There were seven rats in every group. The bilateral fimbria-fornix of brain were transected in the stereotaxic apparatus according to Klein＇s method and bilateral ovaries were ablated according to Singh＇s method in order to establish rat＇s model. Result： In cerebral cortex and hippocampus CA1 of brain of FF group, OVX group and OF group, amounts of green cells of ERa decreased remarkably than that in control group（cortex： 4.71±1.11, 5.29±2.01, 4.42±3.99 vs 9.71±4.53;hippocampus： 6.91±2.63, 7.58±4.16, 5.14±3.80 vs 12.57±3.59, all P〈 0.05） and the amounts of red cells of DACE improved remarkably than that in control group （cortex： 15.78±6.01, 17.63±4.42, 20.00±5.16 vs 5.71±3.14;hippocampus： 15.91±5.12, 12.34±5.07, 17.14±4.45 vs 6.85±1.95, all P〈0.05）, Conclusion： Fimbria/fornix transection or ovariectomy can cause the expression of ERα positive neurons decreasing and DACE positive neurons increasing in cerebral cortex and hippocampus CA1 of rat＇s brain, the combination fimbria/fornix transection and ovariectomy can cause double labeling cells improving in cerebral cortex.

作者金丽英郭宗君崔红波

机构地区青岛大学医学院附属医院脑血管病研究所兖矿集团公司总医院神经内科

出处《中国康复医学杂志》 CAS CSCD 北大核心 2007年第9期782-784,I0001,共4页 Chinese Journal of Rehabilitation Medicine

基金山东省科技厅科研基金(003130110 22130109)

关键词穹窿下器官海马卵巢切除术受体雌激素淀粉样Β蛋白前体 subfornical organ hippocampus ovariectomy receptor, estrogen amyloid beta-protein precursor

分类号 R749.16 [医药卫生—神经病学与精神病学]

引文网络
相关文献

参考文献11

1谢欲晓.认知康复的新领域——认知神经心理康复[J].中国康复医学杂志,2004,19(1):5-5. 被引量：20
2Evin G,Weidemann A.Biogenesis and metabolism of Alzheimer's disease Aβ amyloid peptides[J].Peptides,2002,23(7):1285.
3Singh M,Meyer EM,Millard WJ,et al.Ovarian steroid deprivation results in a reversible learning impairment and compromised cholinergic function in female SD rats[J].Brain Res,1994,644(2):305.
4Krugel U,Bigl V,Eschrich K,et al.Deafferentation of the septo-hippooampal pathway in rats as a model of the metabolic events in Alzheimer's disease[J].Int J Devl Neurosci,2001,19(3):263.
5Paxinos G,Watson C.The rat brain in sterrotaxic coordinates[M].Sydney:Academic Press Australia,1988,23.
6Ostedund M,Kuiper GG,Gusfasson J,et al.Differential distibution and regulation of estrogen receptor alpha and receptor β mRNA with in the female rat brain[J].Brain Res Mol Brain Res,1998,54(1):175.
7Beach TG,Potter PE,Kuo YM,et al.Cholinergic deafferentation of the rabbit codex:A new animal model of Aβ deposition[J].Neurosci Lett,2000,283(1):9.
8Gibbs RB.Levels of trkA and BDNF mRNA,but not NGF mRNA,fluctuate across the estrous cycle and increase in response to acute hormone replacement[J].Brain Res,1998,787(2):259.
9郭宗君,金丽英,杜芳,张伟,姜长青.雌激素对去卵巢大鼠不同脑区淀粉样β蛋白前体蛋白β位点裂解酶表达的影响(英文)[J].中国临床康复,2005,9(36):185-187. 被引量：7
10Funato H,Yoshimura M,Kusui K,et al.Quantition of amyloid β-protein (Aβ) in the cortex during aging and in Alzheimer disease[J].Am J Pathol,1998,152(6):1633.