摘要
目的对茅苍术3-羟基-3-甲基戊二酰辅酶A还原酶(3-hydroxy-3-methylglutaryl-CoA reductase,HMGR)进行基因克隆及序列分析。方法采用cDNA末端快速扩增技术,以茅苍术嫩叶总RNA的cDNA为模板扩增茅苍术HMGR基因的保守区片断。结果序列分析表明,所克隆的cDNA保守区序列长度为458bp,而且同时得到了两个核苷酸序列的同源性为84.28%的片段,相应的氨基酸序列的同源性为92.11%。分别命名为HMGRcr1,HMGR-cr2。推断这可能是该基因家族中的两个成员。而且同源序列比对发现,推断的HMGRcr1氨基酸序列和HMGRcr2氨基酸序列与其他植物都有较高的同源性。结论首次分离并报道了茅苍术HMGRcDNA克隆,为进一步研究萜类生物合成机制及其在提高植物药用价值方面提供理论依据。
Objective To clone and sequence cDNA encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase （HMGR） from Atractylodes lancea. Methods The cDNA, encoding HMGR in A, lancea, was amplified by RACE strategy with the cDNA of the total RNA of young leaves as the template. The partial fragments of HMGR were cloned and sequenced. Results The analysis results revealed that the conserved fragments were 458 bp. At the same time, the two fragments had been obtained 84.28% identification in nucleotide acid and 92.11% identification in corresponding amino acid, named as HMGRcrl and HMGRcr2, respectively. It was deduced that they may be members of the HMGR gene family in A. lancea. Sequencing analysis showed that HMGRcrl and HMGRcr2 had high identity with HMGR from other plants. Conclusion The eDNA encoding HMGR from A. lancea is cloned and reported for the first time. The work will provided a foundation for exploring the mechanism of terpenes biosynthesis and application to the other medicinal plants.
出处
《中草药》
CAS
CSCD
北大核心
2007年第10期1551-1554,共4页
Chinese Traditional and Herbal Drugs
基金
江苏省药用植物重点实验室开放课题资助(02AXL12)
关键词
茅苍术
3-羟基-3-甲基戊二酰辅酶A还原酶
基因克隆
Atractylodes lancea （Thunb.） DC.
3-hydroxy-3-methylglutaryl-eoenzyme A reduetase （HMGR）
gene cloning
