摘要
目的观察黄芪甲苷抗氧化机制对病毒性心肌炎的保护作用。方法腹腔接种CVB3建立小鼠病毒性心肌炎模型,随机分为黄芪甲苷高剂量治疗组[1mg/(kg·d)]、中剂量治疗组[0.5mg/(kg·d)]、低剂量治疗组[0.25mg/(kg·d)]及生理盐水对照组。另设黄芪甲苷高剂量正常对照组及正常空白对照组。分别对小鼠生存率、心肌病理、心肌总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)活性、髓过氧化酶(myeloperoxidase,MPO)及反应性活性氧(reactive oxygen species,ROS)进行检测。结果黄芪甲苷高、中剂量组可明显增加急性期小鼠的生存率及减轻心肌炎症性病理变化,与生理盐水对照组比较差异有统计学意义(P<0.01),还可使心肌T-SOD、GSH-PX、CAT活力增加,MPO及ROS含量降低。结论抗氧化作用可能是黄芪甲苷治疗病毒性心肌炎的重要机制之一。
Objectives To invesitgate the antioxidant effect of Astragaloside in coxsackievirus B （CVB） 3-induced murine myocarditis. Methods Four-week- old Balb/c mice were inoculated intraperitoneally with the Nancy strain of CVB3 and divided into 4 groups, high dose Astragaloside group （1 mg/（kg·d）） medium dose astragaloside group （0.5 mg,/（kg·d））, low dose Astragaloside group （0.25 mg/（kg·d）） and saline control group. In addition, mice inoculated intraperitoneally with non-viral solution were divided into high dose Astragaloside and normal control group. Astragaloside was administered daily for 6 days. Survival rate, myocardial histopathologie changes, total superoxide dimuiase （T- SOD）, glutathioneperoxidase （GSH-PX）catalase （CAT） activity, myeloperoxidase （MPO） and reactive oxygen species （ROS） in cardiomyocytes were determined on Day 14. Results The survival rate on Day 14 was significantly improved in high dose Astragaloside group and medium dose Astragaloside group compared to that of the saline control group （P 〈 0.01 ）. Astragaloside treatment also significantly attenuated histological myocardial lesion, enhanced the myocardial activity of T-SOD, GSH-PX, CAT and reduced the activity of MPO and ROS on Day 14. Conclusions Astragaloside might play a vital role in CVB3-induced murine myocarditis through the inhibition of oxygen radicals and oxidative stress.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2007年第10期825-827,834,共4页
Journal of Clinical Pediatrics
关键词
黄芪甲苷
病毒
心肌炎
氧化应激
Astragaloside
virus
myocarditis
oxidative stress
