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干扰素和异博定逆转乳腺癌细胞耐药作用的研究 被引量:1

Reversal of multidrug-resistance by Hyperthermia combined with Interferonαand Verapamil in breast cancer
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摘要 目的研究α-干扰素和异博定对体外培养的乳腺癌细胞多药耐药的逆转作用。方法MTT法检测各实验组细胞存活率,计算IC50、耐药倍数和逆转倍数;流式细胞术定量检测细胞表面P170的表达。结果α-干扰素与异博定联合应用使乳腺癌细胞耐阿霉素(ADM)的IC50降低为0.32μmol/L,优于单用异博定(1.23μmol/L)、α-干扰素(2.29μmol/L),逆转倍数由7.25和13.49提高到51.88;且P-170表达均有大幅度下降。结论单独用α-干扰素、异博定均可达到部分逆转乳腺癌耐药细胞(MCF-7/ADR)对ADM的耐药的作用,二者联合使用效果更强。 Objectives: To investigate the reversal of multidrug-resistance by Interferon α and Verapamil in breast cancer cell line. Methods: The cell survival rate, ICSO, drug-resistant times and reversal times of different experiment groups were measured by MTT test. The quantitive expression of P170 on the surface of cells were detected by flow cytometry. Results: The ICSO of adriamycin- resistance treated by Interferon α and Verapamil was 0.32 μ mool/L, superior to Verapamil(1.23 μmtool/L) or Interferon α(2.29 μmool/L) alone. The expression of P170 reduced significantly. Conclusions: Partly reversal was found by Intelferon α and Verapamil alone. After the two factors were combined ,the ADM-resistance of MCF-7/ADR cells were more better.
出处 《生命科学仪器》 2007年第9期14-17,共4页 Life Science Instruments
关键词 乳腺癌 Α-干扰素 异博定 逆转耐药 Breast cancer, Interferon α, Verapamil,Reversal ofmultidrug-resistanee
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