摘要
[目的]观察蜂毒素对肝癌细胞BEL-7402的增殖抑制作用。[方法]肝癌细胞BEL-7402体外培养,采用MTT法检测蜂毒素对其增殖抑制作用,流式细胞术测定蜂毒素对细胞增殖核抗原表达和细胞周期的影响。[结果]蜂毒素体外能够抑制BEL-7402肝癌细胞的增殖活性;并抑制细胞增殖核抗原的表达,呈剂量依赖性;干扰细胞周期,使S期细胞增加,G2/M期细胞减少。[结论]蜂毒素具有抑制BEL-7402肝癌细胞增殖的作用,机制可能与抑制细胞增殖核抗原表达和干扰细胞周期有关。
[Objective]To study the inhibition of melittin on proliferation of hepatocellular carcinoma cell line BEL-7402. [Methods]BEL- 7402 cell line was cultured in vitro, and treated with melittin. The proliferation inhibition was detected by MTT assay, The expression of proliferating cell nuclear antigen （PCNA） and cell cycle phase were detected by flow cytometry. [Results] Melittin cound significantly inhibit the growth of BEL-7402 and block up cells progress through G3/M. The percentage of PCNA positive cell was decreased gradually when concentration of melittin was increased. [Conclusions] Melittin cound inhibit the proliferative activity of hepatocellular carcinoma cell line BEL-7402 and reduce of PCNA positive cell expression and arrest cells in S phase.
出处
《浙江中医药大学学报》
CAS
2007年第5期560-561,564,共3页
Journal of Zhejiang Chinese Medical University
基金
上海市科委青年科技启明星计划资助(No:05QMX1471)~~
关键词
蜂毒素
肝细胞癌
增殖
细胞增殖核抗原
细胞周期
Melittin
hepatocellular carcinoma
proliferation
proliferating cell nuclear antigen
cell cycle phase
