摘要
目的研究多瘤病毒的基因载量与异基因造血干细胞移植出血性膀胱炎发生的关系。预防移植后出血性膀胱炎的发生。方法收集40例健康人和40例异基因造血干细胞移植及20例合并出血性膀胱炎患者的血液和尿液，合成多瘤病毒BKV、JCV和SV40基因引物，应用传统的PCR和EvaGreen染料荧光定量PCR法，分别检测血液和尿液多瘤病毒BKV、JCV和SV40DNA。结果40例健康人和40例异基因造血干细胞移植患者血液中BKV、JCV和SV40基因均为阴性；尿液中正常健康人BKV基因的检出率为15％（6／40），JCV基因的检出率为10％（4／40）。异基因造血干细胞移植患者BKV检出率为100％（40／40），JCV基因的检出率为12％（5／40），SV40基因均为阴性。20例出血性膀胱炎患者尿液中BKV基因载量均高于正常人和无出血性膀胱炎的异基因造血干细胞移植患者。结论正常健康人的泌尿系潜伏存在病毒BKV和JCV基因，异基因造血干细胞移植患者出血性膀胱炎的发生与BKV多瘤病毒DNA的载量有关。
Objective To study the correlation between polyoma virus load and hemorrhagic cystitis after allogeneic stem cells transplantation for provide hemorrhagic cystitis. Methods From healthy blood and urine 40 healthy samples,40 patients with stem cells transplantation and 20 patients with hemorrhagic cystitis were detected for VP1 gene of BKV, JCV and SV10 by Polymerase chain reaction and from urine 40 patient with stem cells transplantation samples and 20 patients with hemorrhagic cystitis was quantified for BKV by EvaGreen stain fluorescence quantitative ..Results In the peripheral blood, all gene of BKV, JCV and SV40 were negative, while BKVin urine from healthy and patient with stem cells transplantation was 15% （6/40）and 100% （40/40）, respectively. The JCV was 10 % （4/40）and 12% （5/40）, the SV40 was negative. Conclusion There was BKV and JCV gene from healthy individual and patients urinary series. The load of polyoma virus and incidence of hemorrhagic cystitis is correlation.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2007年第3期244-246,共3页
Chinese Journal of Experimental and Clinical Virology
