摘要
目的:探讨辣椒素治疗实验性变应性鼻炎的机制,进一步研究变应性鼻炎中神经肽与肥大细胞之间的关系。方法:健康SD大鼠30只,随机分为自然对照组、变应性鼻炎组和辣椒素治疗组,每组各10只。用卵清蛋白腹腔注射免疫,继之鼻腔局部免疫建立大鼠变应性鼻炎模型,并进行辣椒素治疗2周,观察各组大鼠行为学改变,取各组动物的鼻黏膜行常规苏木精-伊红染色、甲苯胺蓝染色及免疫组织化学染色,计数并比较肥大细胞在各组之间的差异,观察P物质(SP)在鼻黏膜中分布密度的差异。结果:变应性鼻炎组肥大细胞计数大于自然对照组(P<0.01)。辣椒素治疗组肥大细胞少见,低于变应性鼻炎组(P<0.01);SP的表达低于变应性鼻炎组(P<0.01),但与自然对照组比较差异无统计学意义(P>0.05)。组织中SP的表达与肥大细胞浸润有一定的正相关性(r=0.8252,P<0.05)。结论:辣椒素治疗变应性鼻炎模型可有效减少肥大细胞的浸润,使SP表达减少,各种过敏症状明显改善。
Objective：To explore the relationship between neuropeptides and maste cell in the initiation and development of allergic rhinitis. Method：Thirty healthy rats were randomly divided into three groups. The rat model of allergic rhinitis was established by using ovalbumin intraperitoneal immunizationand nasal antigen challenge. After treating with capsaicin for two weeks, the counts of mast cells and the density of SP distribution were observed routinely in the nasal mucosa obtained from each models by HE, toluidine blue and immunohistochemical staining. Result： The counts of mast cells in AR were greatly more than them in normal controls（ P 〈0.01 ）. After treating with capsaicin the mast cells were rare and significantly fewer than the normals（ P〈0.01 ）;The expression of SP waslower than the AR（ P〈0.01）, but no diference between the capsaicin group and normal group （ P〉0.05）. Conclusion：Capsaicin can decrease the infiltration of mast ceils, downregluate the SP expression, and improve the symptoms of AR greatly.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2007年第20期947-949,共3页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
