摘要
目的建立了测定不同地区川西獐芽菜中的龙胆苦苷。方法采用RP-HPLC法,Kromasil C18色谱柱（250 mm×4.6mm,5μm）,流动相为甲醇-0.04%磷酸水溶液（23：77）,流速1.0 ml·min^-1,检测波长238 nm,柱温35℃。结果龙胆苦苷的线性范围为0.026-1.048μg（r=0.9996）,平均回收率为99.6%,RSD=1.54%。结论所建方法简便、准确、重复性好,适用于川西獐芽菜及其他药用植物中的龙胆苦苷。
OBJECTIVE To establish a method to determine gentiopicroside in Swertia mussotii Franch in different districts. METHODS RP - HPLC was used for the determination of gentiopicroside in Swertia mussotii Franch. The samples were separated on the column of Kromsil C ss （250 mm × 4.6 mm,5 μm） at the column temperature at 30℃, with methanol -water（0.04% phosphoric acid） （23 ： 77 ）as the mobile phase at a flow rate of 1.0 ml· min^-1. The peaks were detect at 238 nm. RESULTS The gentiopicroside had a good linearity in the range of 0. 026 - 1. 048 μg （ r = 0.9996 ） and the average recovery was 99.6 % with RSD of 1.54%. CONCLUSION The method is simple, accurate and repeatable . It can be suitable for the determination of gentiopicroside in Swertia mussotii Franch and other plants.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2007年第5期564-565,共2页
West China Journal of Pharmaceutical Sciences
基金
教育部科学技术重点研究项目(项目编号:205167)
关键词
藏药
川西獐芽菜
龙胆苦苷
反相高效液相色谱法
Tibetan drug
Swertia mussotii Franch
Gentiopicroside
Reversed -phase high performance liquid chromatography
