摘要
目的采用毛细管气相色谱法同时测定复方牙痛酊中樟脑和乙酸龙脑酯的含量,建立复方牙痛酊质量控制体系。方法以水杨酸甲酯为内标,石英毛细管柱OV-1701（30 m×0.32 mm,0.25μm）为固定相,氮气为载气,FID检测器。结果在该色谱条件下,乙酸龙脑酯和樟脑分别在0.0295-0.295 mg·ml^-1,0.012 72-0.127 2 mg·ml^-1的浓度范围内线性关系良好（r〉0.999 5）;平均回收率分别为97.2%,98.1%,RSD分别为2.35%,2.14%（n=6）。结论该方法简单、灵敏、快速、回收率和重现性好,可以作为复方牙痛酊的质量控制标准。
Objective This paper reports a GC method for determination of bornyl acetate and camphor in Fufangyatongding by gas chromatography. Methods Methyl salicylate has been used as the internal standard substance in internal standard method. The GC system consisted of Capillary column, OV - 1701 （30 m × 0.32 mm × 0.25 μm）as the stationary phase, nitrogen as the carrier gas, and FID as the detector. Results Both bornyl acetate and camphor got a good linearity between 0.0295 - 0.295 mg·ml^-1, 0.01272 - 0. 1272 mg·ml^-1 under the chromatographic condition. The mean recovery of bornyl acetate and camphor were 97.2% ,98.1% ,and RSD were 2.35% ,2.14% （n =6）. Conclusion The method is simple, sensitive and rapid with satisfactory recoveries and reproducibilities.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第10期2458-2459,共2页
Lishizhen Medicine and Materia Medica Research
