摘要
目的:探讨ZnPcS2P2在K562细胞,HL-60细胞亚细胞结构中的精确定位,揭示光动力学疗法(photody-namic therapy,PDT)的作用机制。方法:将K562细胞,HL-60细胞与ZnPcS2P2共同孵育5 h。应用激光扫描共聚焦显微成像系统,选择特异性细胞器荧光探针(线粒体探针若丹明Rodanmine123、溶酶体探针LysoTrackerDND-26、内质网探针Dioc6(3)采用波形比较法对光敏剂进行亚细胞定位。结果:ZnPcS2P2在K562细胞,HL-60细胞中发出的荧光与负载的Rodanmine123、Lyso-TracKer DND-26、Dioc6(3)均有部分重叠,波形均有相似之处。ZnPc-S2P2在线粒体、溶酶体、内质网均有分布。结论:线粒体是ZnPcS2P2介导的PDT(ZnPcS2P2-PDT)光损伤的主要靶点,溶酶体、内质网也是ZnPcS2P2-PDT光损伤的靶点。
Objective. To investigate the mechanism of a novel amphipathic photo sensitizer [ di-sulfonated di-phthalimidomethyl phthalocyanine zinc （ZnPcS2P2 ）]-mediated photodynamic therapy （ZnPcSEPE-PDT） on K562 cells and HL60 cells. Methods： Laser scanning confocal microscopy was applied and three special organelle probes including Rhodamine123, Lys -oTracker DND-26 and Dioc6 （3） were selected to label mitochondria, lysosome and endoplasmic reticulum fluorescence images both of ZnPc-SEP2 and the three probes were collected through corresponding emission filters. Results： ZnPcSEP2 was located on mitochondria lysosome, endoplasmic reticulum. Conclusion： This study provides direct evidence that mitochondria, lysosome and endoplasmic reticulum play an important role in the apoptosis in K562 cells and HL60 cells induced by ZnPcSEP2- PDT.
出处
《激光生物学报》
CAS
CSCD
2007年第5期572-575,共4页
Acta Laser Biology Sinica
基金
福建省科技厅基金项目(2004y020)
关键词
激光共聚焦
光敏剂
亚细胞定位
laser scanning confocus
photosensitizer
subcellular location
