摘要
以毛竹(Phyllostachys edulis)干种子为材料,采用室内发芽实验、电导率测定和TTC活力测定以及早期幼苗生长分级等4种方法研究了60Coγ射线对种子活力及早期幼苗生长的诱导效应。结果表明:当辐照剂量低于100Gy时,辐照能加快种子萌发进程,提高发芽率;而当剂量超过100Gy,则对毛竹种胚组织造成损伤,抑制细胞生长和分裂,不同程度地延迟了毛竹种子发芽过程,降低了种子活力,造成早期苗高和根生长受到明显抑制,抑制作用随辐照剂量的增大而增强。毛竹种子发生诱变效应的最佳辐照剂量范围为100~175Gy。24h电导率与发芽率、发芽指数、活力指数、苗高和根生长等活力指标间存在极显著线性关系,是快速检验毛竹种子活力的重要指标。24h电导率和苗高又可作为检查辐射后毛竹种子活力变化和发生诱导效应的有效指标。
The dry seeds of Phyllostachys edulis were irradiated by different doses of 60^Coγ rays, the effects of the radiation on seed vigor and seedling growth characters were investigated by four testing methods, i. e. germination testing indoor, electrical conductivity, TTC vigor testing and growth classification of seedling. Results showed that the germination process and germination rate could be accelerated by doses of 60^Coγ rays（ ≤ 100Gy）, and higher doses of 60^Coγ rays（ 〉 100Gy） could obviously inhibit the germination process and reduce seed vigor, while induse seed embryo broken, cell division, growth restrained, the height of young seedling and length of root decreasing. The inhibition effects were significantly increased with radiation dose increase. The optimal range of radiation dose for radiation breeding of Phyllostachys edulis dry seeds was 100 to 175Gy. Linear relationships were existed in electrical conductivity after dipping in water for 24h in germination rate（G）, germination index （GI）, vigor index （Ⅵ）, height of seedlings and length of root. EC after 24h and height of seedlings were chosen to test the change of seeds vigor and the effect of the radiation of 60^Coγ rays on Phyllostachys edulis.
出处
《核农学报》
CAS
CSCD
北大核心
2007年第5期436-440,455,共6页
Journal of Nuclear Agricultural Sciences
基金
国家"十一五"科技支撑项目资助(2006BAD19B0203)
关键词
60^Coγ射线
毛竹
辐射剂量
种子活力
萌发
60^Coγ rays
Phyllostachys edulis
irradiation dose
seed vigor
germination
