摘要
目的:探讨凉血化瘀方防治急性肝衰竭的最佳配伍。方法:ICR小鼠85只被随机分成17组,包括正常组和16个用药组,根据L16(215)正交表将凉血化瘀方按四因素两水平安排成16种不同的搭配,制成煎剂分别灌胃给药,每组小鼠腹腔注射D-GaIN(500mg/kg)+LPS(4.8μg/kg)造成急性肝功能衰竭,6小时后取血和肝组织,观察不同药物组小鼠的胆红素(TBil)、转氨酶(ALT、AST)水平和肝组织病理损伤程度。结果:赤芍、生地、大黄配伍组比模型组和其他用药组能够显著降低肝衰竭小鼠胆红素水平,减轻肝组织坏死程度。结论:凉血化瘀中药具有很好的防治肝衰竭,保护肝细胞的作用,且由赤芍、生地、大黄配伍组方疗效最佳。
Objective., To study the best compatibility of cooling blood medicines in fulminant hepatic failure. Methods： Eighty five ICR mice were randomly divided into 17 groups, including control group and 16 different combinations. L16 （2^15） orthogonal table was used to arrange formula with the function of cooling blood to remove blood stasis into 16 different combinations. The decoctions of the 16 different combinations were given to the mice by ig. Each group of mice was injected D-GalN （500mg/kg）＋LPS （4.8μg/kg） to cause FHF, 6 hours later, the blood and hepatic tissue were collected, the level of bilirubin, AST, ALT and the degree of hepatic lesion in different groups of mice. were observed. Results： The formula of Chishao, Dahuang and Shengdi had notable effect on reducing bilirubin and relieving the patho- logical lesion of hepatic tissue. Conclusion： The compatibility of cooling blood medicines has good curative effect on FHF. The formula of Chishao, Dahunag and Shengdi is the best one.
出处
《中西医结合肝病杂志》
CAS
2007年第5期281-283,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
国家中医药管理局中医药科技专项课题资助(No.04-05JP27)
关键词
急性肝衰竭
方剂
凉血
化瘀
正交设计
fulminant hepatic failure
formula
cooling blood
remove blood stasis
orthogonal experimental design
