摘要
目的:探讨高压氧(hyperbaric oxygen HBO)治疗对兔蛛网膜下腔出血(SAH)后迟发性脑血管痉挛(DCVS)模型脑血管痉挛程度的影响,及其对该模型血清一氧化氮(NO)、血清一氧化氮合酶(NOS)含量,以及脑组织中水、K+、Na+、Ca2+含量的影响。方法:枕大池二次注血法建立迟发性脑血管痉挛模型,将动物分为SAH组和SAH+HBO组,用显微测量法测量基底动脉管径,用比色法测定血清中NO及NOS含量,用干湿比较法测定不同时间脑组织水含量,用原子吸收分光光度计测定脑组织K+、Na+、Ca2+含量。结果:SAH组于模型制作成功后24h、96h脑血管直径变小,脑组织水含量和Na+含量显著升高,脑组织K+含量减低,钙含量明显增加;SAH+HBO上述指标异常改变均较小。结论:高压氧治疗能够缓解SAH后脑水肿和脑损伤,能够缓解迟发性脑血管痉挛的程度。
Objectives：To investigate the effects of hyperbaric oxygen （ HBO ） on the diameter of the basilar artery, the plasma NO and serum NOS levels,brain water and electrolytic content in delay cerebral vasospasm rabbit model following subarachnoid hemorrhage （SAH）. Methods：The rabbit model of SAH was built by two-time injection of blood via cistern after the bilateral carotid was occluded. The animals were randomly divided into SAH group and SAH＋HBO group. The diameter of the basilar artery was measured with microscope, the serum NO and serum NOS were detected by colorimetric method, brain water content was determined by dry and wet weight comparison, brain sodium, potassium, and calcium levels were detected by atomic absorption spectrophotometry. Results：In SAH group,the diameter of the basilar artery at 96h became smaller than that in the baseline, the serum NO and serum NOS levels at 24h and 96h were lower than those in the baseline,the brain water content and sodium content in SAH group at 24h and 96h were significantly higher than those in the baseline,the brain potassium content was lower than that in the baseline,the brain calcium content was higher than that in the baseline. The alterations of the above objects in SAH＋HBO group were not as obvious as those in the SAH group. Conclusion：HBO may relieve cerebral vasospasm（CVS）, brain edema and secondary cerebral injury caused by SAH.
出处
《黑龙江医药科学》
2007年第5期36-37,共2页
Heilongjiang Medicine and Pharmacy
