摘要
目的探讨散发神经鞘瘤病人22号染色体(CHR22)杂合子丢失(LOH)情况及其与肿瘤增殖的关系。方法选取4个与NF2基因密切相关的多态性微卫星标记物,应用PCR方法研究54例神经鞘瘤的LOH情况。采用Ki-67和增殖细胞核抗原(PCNA)评估增殖指数。结果23例(42.6%)CHR22发生LOH,听神经鞘瘤发生率显著性高于脊神经鞘瘤(χ2=5.14,P<0.05)。发生LOH者增殖指数明显高于无LOH者(Pki-67=0.0079,PPCNA=0.0021)。结论在散发神经鞘瘤中,CHR22LOH是常发事件;听神经瘤与脊神经鞘瘤LOH发生率有显著性差异,CHR22LOH与神经鞘瘤的增殖活动性有关。
Objective To explore the loss of heterozygosity （LOH） on chromosome 22 （CHR22） in patients with sporadic schwannoma and the correlation with tumor proliferation. Methods Four highly polymorphic microsatellite DNA markers closely related to NF2 gene were used to analyze the frequency of LOH in 54 schwannomas by polymerase chain reaction. The proliferation index was evaluated by Ki-67 and proliferative cell nuclear antigen （PCNA）. Results Twenty-three （42.6%） schwannomas showed LOH. The frequency of LOH in vestibular schwannoma was significantly higher than that in spinal schwannoma （χ2 = 5.14 ,P 〈 0.05）. The proliferation index of schwannoma with LOH was significantly higher than that without LOH （Pki-67 = 0.007 9, PPCNA = 0.002 1）. Conclusion LOH on chromosome 22 is a frequent event in the tumorigenesis of sporadic schwannoma. The frequency of LOH in vestibular schwannoma is significantly different from that in spinal schwannoma, And there is a correlation between LOH on chromosome 22 and proliferative activity in schwannoma.
出处
《中国微侵袭神经外科杂志》
CAS
2007年第10期454-456,共3页
Chinese Journal of Minimally Invasive Neurosurgery
关键词
神经鞘瘤
杂合子丢失
染色体
人
22对
neurilemmoma
loss ofheterozygosity
chromosomes, human, pair 22
