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血管活性肠肽对暂时性脑缺血大鼠梗死体积的影响 被引量：2

Effects of vasoactive intestinal peptide on the infarct volume in rat transient focal cerebral Ischemia
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摘要 目的通过活体实验研究血管活性肠肽(VIP)对脑缺血损伤的神经保护作用。方法大鼠侧脑室内注射VIP后,采用大脑中动脉线栓(MCAO)法诱导建立暂时性局部脑缺血模型。通过神经学的评价对MCAO大鼠进行判定和筛选;TTC染色测定大鼠脑梗死体积;双抗夹心ELISA检测血清S100β的含量。结果MCAO后2 h大鼠呈现不同程度的神经功能损伤症状。评定为1-2级的仅有2例出现明显脑梗死区,3级的为全部。VIP注射后大鼠脑梗死体积明显缩小,较对照组减少约25%(P<0.05);血清S100β浓度也明显降低(P<0.05)。结论以神经学评分3级作为MCAO模型成功的判定标准是较为可靠的。VIP脑内注射可明显减小脑缺血后梗死体积,具有神经保护作用。VIP可以降低S100β的含量,这可能涉及到VIP的另一神经保护机制。 Objective To explore the neuroprotective effects of vasoactive intestinal peptide （VIP） on ischemic brain injury in vivo. Methods In the present study, VIP was given via intracerebroventriclar injection, and then focal cerebral ischemia was induced in SD rats by intraluminal occlusion of middle cerebral artery （MACO） for 120 min. The evaluation and screening of rats with successful occlusion of MCA were performed by neurological testing. The infarct volume was investigated with 2, 3, 5-triphenyltetrazolium chloride （TTC） stain. S100fl concentration in serum was detected by double-antibodies-sandwich-ELISA method. Results The different grades of neurological severity appeared obviously in all the rats at 2 hours after MCAO. Only two rats that scored grade 1 or 2 had identifiable areas of infarction, whereas the areas of infarction in all the rats that were scored grade 3 were identified. Compared to the control groups at 1 day, injection of VIP significantly reduced the relative infarct volume by approximately 25% （P〈0. 05）, and then remarkably decreased S100 β serum concentration （P〈0.05）. Conclusion The grade 3 of neurological score as a standard to evaluate successful occlusion of MCA is satisfactory and reliable. VIP injection can reduce the infarct volume in a rat model of focal cerebral ischemia. The present study provides further evidence for the neuroprotective effects of VIP in vivo. VIP may decrease S100 β serum concentration, which may involve one of the mechanisms of the neuroprotective effect of VIP.
作者 赵朝华 胡晓丹 杨杰 钱亦华 邱芬 刘勇
机构地区 西安交通大学医学院人体解剖与组织胚胎系
出处 《西安交通大学学报（医学版）》 CAS CSCD 北大核心 2007年第5期494-497,共4页 Journal of Xi’an Jiaotong University（Medical Sciences）
基金 国家自然科学基金资助项目(No.30570976)
关键词 血管活性肠肽 脑缺血 梗死体积 S100Β vasoactive intestinal peptide cerebral ischemia infarct volume S100 β
分类号 R743 [医药卫生—神经病学与精神病学]
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参考文献10

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共引文献6

同被引文献52

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二级引证文献5

西安交通大学学报（医学版）
2007年 第5期

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