摘要
本文建立了非水体系高效毛细管电泳法测定芬布芬的新方法。考察了运行电压、非水介质和电解质等因素的影响。选择15mmol／L NaAc-25mmol／L十六烷基三甲基溴化铵（CTAB）为电泳介质，甲醇为溶剂，紫外检测波长281nm，分离电压-25kV，13min内可以实现芬布芬的分离检测。在优化条件下，药物中辅料不干扰芬布芬的测定，加标回收率为98．2％～103．1％。方法简便、快速，可用于芬布芬片中芬布芬含量的测定。
The new method for the determination of Fenbufen in Fenbufen tablets by non-aqueous capillary electrophoresis （NACE） was developed. The determination conditions were as follows. Methanol buffer medium containing 15 mmol/L sodium acetate-25 mmol/L hexadecyltrimethylam monium（CTAB） was used as runing buffer; the applied voltage was -25. 0 kV, and the detection wavelength was 281 nm. Under these NACE conditions, Fenbufen could be separated within 13 min. Linear calibration curve was obtained in the range of 0. 912 - 18. 24 μg/mL for parecetamol with a correlation coefficient of 0. 9995. Detection limit was 0.18 μg/mL. The developed method was applied to the determination of parecetamol in tablet with recoveries ranging from 98.2 %- 103.1 %.
出处
《分析科学学报》
CAS
CSCD
2007年第5期551-554,共4页
Journal of Analytical Science
基金
广西自然科学基金(桂科自No.0146001)
广西教育厅科学研究基金(桂教科[2000]392)
关键词
毛细管电泳
芬布芬
非水相体系
Fenbufen
Non-aqueous system
Capillary electrophoresis
